Prevention and treatment of allergic inflammation by an Fcγ-Der f2 fusion protein in a murine model of dust mite-induced asthma

Li Hui Lin, Ping Zheng, Wai Man Yuen, Juan Wang, Juan Zhou, Cun Quan Kong, Xia Peng, Jia Li, Li Li

Research output: Journal article publicationJournal articleAcademic researchpeer-review

12 Citations (Scopus)


The immunoglobulin E (IgE) high-affinity receptor FcεRI expressed on mast cells and basophils plays a critical role in triggering allergic disease. The co-aggregation of the FcεRI and FcγRIIb receptors is inhibitory to FcεRI signaling and holds great potential for the treatment of IgE-mediated allergies. In China, Dermatophagoides farinae is a common anaphylaxis trigger. Therefore, in this study, the FcγRIIb-mediated immunomodulating activity of recombinant Fcγ-Der f2 fusion protein was tested in a Der f2-allergic murine model. Following the treatment, bronchoalveolar lavage fluid (BALF) was collected to measure the expression of several Th1/Th2-type cytokines (IL-5, TNF-α, IL-12p70, IL-4, IL-10, IFN-γ and IL-18) and histamine, while blood was used to detect the specific IgE and IgG-types anti-Der f2 antibodies, for measurement. In contrast to the saline-treated allergic mice, the levels of Der f2-specific IgE, cytokines and histamine were lowered in the Fcγ-Der f2-treated allergic mice, in addition to the rare inflammatory cell infiltration in the airways and blood vessels revealed by histopathological examination. The recombinant Fcγ-Der f2 protein was demonstrated to function as an effective immunotherapeutic agent, suggesting that chimeric human Fcγ-allergen proteins could be used in the development of antigen-specific immunotherapy for human allergic diseases.
Original languageEnglish
Pages (from-to)276-283
Number of pages8
JournalImmunologic Research
Issue number3
Publication statusPublished - 1 Jun 2012


  • Allergic asthma mouse
  • Fcγ-Der f2 fusion protein
  • FcγRIIb
  • FcεRI
  • Immunomodulation

ASJC Scopus subject areas

  • Immunology


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