Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan

Patrick Chi Leung Hui; Wen Yi Wang; Chi Wai Kan; Frency Sau Fun Ng; Chang E. Zhou; Elaine Wat; Vanilla Xin Zhang; Chung Lap Chan; Clara Bik San Lau; Ping Chung Leung

doi:10.1016/j.colsurfa.2013.05.043

Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan

Patrick Chi Leung Hui, Wen Yi Wang, Chi Wai Kan, Frency Sau Fun Ng, Chang E. Zhou, Elaine Wat, Vanilla Xin Zhang, Chung Lap Chan, Clara Bik San Lau, Ping Chung Leung

The Hong Kong Polytechnic University

Research output: Journal article publication › Journal article › Academic research › peer-review

35 Citations (Scopus)

Abstract

CSA microcapsules were prepared to encapsulate Cortex Moutan using emulsion-chemical cross-linking method and the optimum preparation conditions were studied by orthogonal array analysis. Based on encapsulation efficiency, the optimum preparation conditions for CSA microcapsule were: core-shell ratio = 1:2; shell concentration = 6% (w/v); agitation speed = 1100. rpm and cross-linking time = 60. min. The microcapsules were characterised by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and laser diffraction particle size analysis. The in vitro drug release behaviour of Cortex Moutan from the microcapsules was studied by HPLC. The in vitro cytotoxicity test was studied by MTT assay and LDH release assay. Results showed that the microcapsules were in spherical form with diameter mostly in the range of 1-10. μm. The FTIR spectra indicated that Cortex Moutan was successfully encapsulated in the microcapsules. The experimental results showed that the release performance of the microcapsules was influenced by pH value of PBS and the cytotoxicity test showed that the microcapsules had no toxic effect on cells. These results indicate that Cortex Moutan loaded CSA microcapsule may possess potential application in clinical treatment of atopic dermatitis.

Original language	English
Pages (from-to)	95-101
Number of pages	7
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	434
DOIs	https://doi.org/10.1016/j.colsurfa.2013.05.043
Publication status	Published - 5 Oct 2013

Keywords

Atopic dermatitis
Cortex Moutan
Cytotoxicity test
Microencapsulation

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

More information

10.1016/j.colsurfa.2013.05.043

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Hui, P. C. L., Wang, W. Y., Kan, C. W., Ng, F. S. F., Zhou, C. E., Wat, E., Zhang, V. X., Chan, C. L., Lau, C. B. S., & Leung, P. C. (2013). Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 434, 95-101. https://doi.org/10.1016/j.colsurfa.2013.05.043

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title = "Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan",

abstract = "CSA microcapsules were prepared to encapsulate Cortex Moutan using emulsion-chemical cross-linking method and the optimum preparation conditions were studied by orthogonal array analysis. Based on encapsulation efficiency, the optimum preparation conditions for CSA microcapsule were: core-shell ratio = 1:2; shell concentration = 6% (w/v); agitation speed = 1100. rpm and cross-linking time = 60. min. The microcapsules were characterised by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and laser diffraction particle size analysis. The in vitro drug release behaviour of Cortex Moutan from the microcapsules was studied by HPLC. The in vitro cytotoxicity test was studied by MTT assay and LDH release assay. Results showed that the microcapsules were in spherical form with diameter mostly in the range of 1-10. μm. The FTIR spectra indicated that Cortex Moutan was successfully encapsulated in the microcapsules. The experimental results showed that the release performance of the microcapsules was influenced by pH value of PBS and the cytotoxicity test showed that the microcapsules had no toxic effect on cells. These results indicate that Cortex Moutan loaded CSA microcapsule may possess potential application in clinical treatment of atopic dermatitis.",

keywords = "Atopic dermatitis, Cortex Moutan, Cytotoxicity test, Microencapsulation",

author = "Hui, {Patrick Chi Leung} and Wang, {Wen Yi} and Kan, {Chi Wai} and Ng, {Frency Sau Fun} and Zhou, {Chang E.} and Elaine Wat and Zhang, {Vanilla Xin} and Chan, {Chung Lap} and Lau, {Clara Bik San} and Leung, {Ping Chung}",

note = "Funding Information: We gratefully acknowledge financial support from Innovation and Technology Fund of the Government of the Hong Kong Special Administrative Region (Project Code: ITS/409/09) and the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. PolyU 5171/10E).",

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doi = "10.1016/j.colsurfa.2013.05.043",

language = "English",

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Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan. / Hui, Patrick Chi Leung; Wang, Wen Yi ; Kan, Chi Wai et al.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 434, 05.10.2013, p. 95-101.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan

AU - Hui, Patrick Chi Leung

AU - Wang, Wen Yi

AU - Kan, Chi Wai

AU - Ng, Frency Sau Fun

AU - Zhou, Chang E.

AU - Wat, Elaine

AU - Zhang, Vanilla Xin

AU - Chan, Chung Lap

AU - Lau, Clara Bik San

AU - Leung, Ping Chung

N1 - Funding Information: We gratefully acknowledge financial support from Innovation and Technology Fund of the Government of the Hong Kong Special Administrative Region (Project Code: ITS/409/09) and the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. PolyU 5171/10E).

PY - 2013/10/5

Y1 - 2013/10/5

N2 - CSA microcapsules were prepared to encapsulate Cortex Moutan using emulsion-chemical cross-linking method and the optimum preparation conditions were studied by orthogonal array analysis. Based on encapsulation efficiency, the optimum preparation conditions for CSA microcapsule were: core-shell ratio = 1:2; shell concentration = 6% (w/v); agitation speed = 1100. rpm and cross-linking time = 60. min. The microcapsules were characterised by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and laser diffraction particle size analysis. The in vitro drug release behaviour of Cortex Moutan from the microcapsules was studied by HPLC. The in vitro cytotoxicity test was studied by MTT assay and LDH release assay. Results showed that the microcapsules were in spherical form with diameter mostly in the range of 1-10. μm. The FTIR spectra indicated that Cortex Moutan was successfully encapsulated in the microcapsules. The experimental results showed that the release performance of the microcapsules was influenced by pH value of PBS and the cytotoxicity test showed that the microcapsules had no toxic effect on cells. These results indicate that Cortex Moutan loaded CSA microcapsule may possess potential application in clinical treatment of atopic dermatitis.

AB - CSA microcapsules were prepared to encapsulate Cortex Moutan using emulsion-chemical cross-linking method and the optimum preparation conditions were studied by orthogonal array analysis. Based on encapsulation efficiency, the optimum preparation conditions for CSA microcapsule were: core-shell ratio = 1:2; shell concentration = 6% (w/v); agitation speed = 1100. rpm and cross-linking time = 60. min. The microcapsules were characterised by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and laser diffraction particle size analysis. The in vitro drug release behaviour of Cortex Moutan from the microcapsules was studied by HPLC. The in vitro cytotoxicity test was studied by MTT assay and LDH release assay. Results showed that the microcapsules were in spherical form with diameter mostly in the range of 1-10. μm. The FTIR spectra indicated that Cortex Moutan was successfully encapsulated in the microcapsules. The experimental results showed that the release performance of the microcapsules was influenced by pH value of PBS and the cytotoxicity test showed that the microcapsules had no toxic effect on cells. These results indicate that Cortex Moutan loaded CSA microcapsule may possess potential application in clinical treatment of atopic dermatitis.

KW - Atopic dermatitis

KW - Cortex Moutan

KW - Cytotoxicity test

KW - Microencapsulation

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U2 - 10.1016/j.colsurfa.2013.05.043

DO - 10.1016/j.colsurfa.2013.05.043

M3 - Journal article

AN - SCOPUS:84878937369

SN - 0927-7757

VL - 434

SP - 95

EP - 101

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

ER -

Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan

Abstract

Keywords

ASJC Scopus subject areas

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