Preparation and characterization of chitosan/sodium alginate (CSA) microcapsule containing Cortex Moutan

Chi Leung Hui, Wen Yi Wang, Chi Wai Kan, Frency Sau Fun Ng, Chang E. Zhou, Elaine Wat, Vanilla Xin Zhang, Chung Lap Chan, Clara Bik San Lau, Ping Chung Leung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

24 Citations (Scopus)

Abstract

CSA microcapsules were prepared to encapsulate Cortex Moutan using emulsion-chemical cross-linking method and the optimum preparation conditions were studied by orthogonal array analysis. Based on encapsulation efficiency, the optimum preparation conditions for CSA microcapsule were: core-shell ratio = 1:2; shell concentration = 6% (w/v); agitation speed = 1100. rpm and cross-linking time = 60. min. The microcapsules were characterised by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and laser diffraction particle size analysis. The in vitro drug release behaviour of Cortex Moutan from the microcapsules was studied by HPLC. The in vitro cytotoxicity test was studied by MTT assay and LDH release assay. Results showed that the microcapsules were in spherical form with diameter mostly in the range of 1-10. μm. The FTIR spectra indicated that Cortex Moutan was successfully encapsulated in the microcapsules. The experimental results showed that the release performance of the microcapsules was influenced by pH value of PBS and the cytotoxicity test showed that the microcapsules had no toxic effect on cells. These results indicate that Cortex Moutan loaded CSA microcapsule may possess potential application in clinical treatment of atopic dermatitis.
Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume434
DOIs
Publication statusPublished - 5 Oct 2013

Keywords

  • Atopic dermatitis
  • Cortex Moutan
  • Cytotoxicity test
  • Microencapsulation

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

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