Prenylated isoflavonoids-rich extract of erythrinae cortex exerted bone protective effects by modulating gut microbial compositions and metabolites in ovariectomized rats

Hui Hui Xiao; Xueli Yu; Chen Yang; Chi On Chan; Lu Lu; Sisi Cao; Siu Wai Wan; Ze Jun Lan; Daniel Kam Wah Mok; Sheng Chen; Mansau Wong

doi:10.3390/nu13092943

Prenylated isoflavonoids-rich extract of erythrinae cortex exerted bone protective effects by modulating gut microbial compositions and metabolites in ovariectomized rats

Hui Hui Xiao, Xueli Yu, Chen Yang, Chi On Chan, Lu Lu, Sisi Cao, Siu Wai Wan, Ze Jun Lan, Daniel Kam Wah Mok, Sheng Chen, Mansau Wong

Research output: Journal article publication › Journal article › Academic research › peer-review

21 Citations (Scopus)

Abstract

Flavonoids, found in a wide variety of foods and plants, are considered to play an important role in the prevention and treatment of osteoporosis. Our previous studies demonstrated that Erythrina cortex extract (EC) rich in prenylated isoflavonoids exerted bone protective effects in ovarectomized (OVX) rats. The present study aimed to investigate the interactions of gut microbiota with the EC extract to explore the underlying mechanisms involved in its beneficial effects on bone. Sprague-Dawley female rats of 3-months-old were ovariectomized and treated with EC extract for 12 weeks. EC extract reversed ovariectomy-induced deterioration of bone mineral density and bone microarchitecture as well as downregulated cathepsin K (Ctsk) and upregulated runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) in the tibia of OVX rats. Its protective effects on bone were correlated with changes in microbial richness and the restorations of several genera. EC increased the serum circulating levels of acetate and propionate in OVX rats. We conclude that the bone protective effects of EC extract were associated with the changes in microbial compositions and serum short chain fatty acids (SCFAs) in OVX rats.

Original language	English
Article number	2943
Journal	Nutrients
Volume	13
Issue number	9
DOIs	https://doi.org/10.3390/nu13092943
Publication status	Published - Sept 2021

Keywords

Erythrinae cortex
Gut microbiota
Ovariec-tomy rats
Prenylated flavonoids
Short chain fatty acids

ASJC Scopus subject areas

Food Science
Nutrition and Dietetics

More information

10.3390/nu13092943

http://hdl.handle.net/10397/95497

Cite this

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title = "Prenylated isoflavonoids-rich extract of erythrinae cortex exerted bone protective effects by modulating gut microbial compositions and metabolites in ovariectomized rats",

abstract = "Flavonoids, found in a wide variety of foods and plants, are considered to play an important role in the prevention and treatment of osteoporosis. Our previous studies demonstrated that Erythrina cortex extract (EC) rich in prenylated isoflavonoids exerted bone protective effects in ovarectomized (OVX) rats. The present study aimed to investigate the interactions of gut microbiota with the EC extract to explore the underlying mechanisms involved in its beneficial effects on bone. Sprague-Dawley female rats of 3-months-old were ovariectomized and treated with EC extract for 12 weeks. EC extract reversed ovariectomy-induced deterioration of bone mineral density and bone microarchitecture as well as downregulated cathepsin K (Ctsk) and upregulated runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) in the tibia of OVX rats. Its protective effects on bone were correlated with changes in microbial richness and the restorations of several genera. EC increased the serum circulating levels of acetate and propionate in OVX rats. We conclude that the bone protective effects of EC extract were associated with the changes in microbial compositions and serum short chain fatty acids (SCFAs) in OVX rats.",

keywords = "Erythrinae cortex, Gut microbiota, Ovariec-tomy rats, Prenylated flavonoids, Short chain fatty acids",

author = "Xiao, {Hui Hui} and Xueli Yu and Chen Yang and Chan, {Chi On} and Lu Lu and Sisi Cao and Wan, {Siu Wai} and Lan, {Ze Jun} and Mok, {Daniel Kam Wah} and Sheng Chen and Mansau Wong",

note = "Funding Information: Funding: This research was funded by the Shenzhen Basic Research Program (JCYJ20170818104152702) and National Natural Science Foundation of China (81903616). The Hong Kong Midstream Research Programme for Universities (MRP/012/18X), General Research Fund (Project No. 15302718) and Collaborative Research Fund (CRF Grant No. C5031-14E, C5033-19E) of the Research Grant Council of the Hong Kong Special Administrative Region, the Hong Kong Chinese Materia Medica Standards Project, Hong Kong Polytechnic University financial support (Project No. YBU0), the Large Equipment Funds and University Research Facility in Chemical and Environmental Analysis and Life Sciences of the Hong Kong Polytechnic University. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = sep,

doi = "10.3390/nu13092943",

language = "English",

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T1 - Prenylated isoflavonoids-rich extract of erythrinae cortex exerted bone protective effects by modulating gut microbial compositions and metabolites in ovariectomized rats

AU - Xiao, Hui Hui

AU - Yu, Xueli

AU - Yang, Chen

AU - Chan, Chi On

AU - Lu, Lu

AU - Cao, Sisi

AU - Wan, Siu Wai

AU - Lan, Ze Jun

AU - Mok, Daniel Kam Wah

AU - Chen, Sheng

AU - Wong, Mansau

N1 - Funding Information: Funding: This research was funded by the Shenzhen Basic Research Program (JCYJ20170818104152702) and National Natural Science Foundation of China (81903616). The Hong Kong Midstream Research Programme for Universities (MRP/012/18X), General Research Fund (Project No. 15302718) and Collaborative Research Fund (CRF Grant No. C5031-14E, C5033-19E) of the Research Grant Council of the Hong Kong Special Administrative Region, the Hong Kong Chinese Materia Medica Standards Project, Hong Kong Polytechnic University financial support (Project No. YBU0), the Large Equipment Funds and University Research Facility in Chemical and Environmental Analysis and Life Sciences of the Hong Kong Polytechnic University. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/9

Y1 - 2021/9

N2 - Flavonoids, found in a wide variety of foods and plants, are considered to play an important role in the prevention and treatment of osteoporosis. Our previous studies demonstrated that Erythrina cortex extract (EC) rich in prenylated isoflavonoids exerted bone protective effects in ovarectomized (OVX) rats. The present study aimed to investigate the interactions of gut microbiota with the EC extract to explore the underlying mechanisms involved in its beneficial effects on bone. Sprague-Dawley female rats of 3-months-old were ovariectomized and treated with EC extract for 12 weeks. EC extract reversed ovariectomy-induced deterioration of bone mineral density and bone microarchitecture as well as downregulated cathepsin K (Ctsk) and upregulated runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) in the tibia of OVX rats. Its protective effects on bone were correlated with changes in microbial richness and the restorations of several genera. EC increased the serum circulating levels of acetate and propionate in OVX rats. We conclude that the bone protective effects of EC extract were associated with the changes in microbial compositions and serum short chain fatty acids (SCFAs) in OVX rats.

AB - Flavonoids, found in a wide variety of foods and plants, are considered to play an important role in the prevention and treatment of osteoporosis. Our previous studies demonstrated that Erythrina cortex extract (EC) rich in prenylated isoflavonoids exerted bone protective effects in ovarectomized (OVX) rats. The present study aimed to investigate the interactions of gut microbiota with the EC extract to explore the underlying mechanisms involved in its beneficial effects on bone. Sprague-Dawley female rats of 3-months-old were ovariectomized and treated with EC extract for 12 weeks. EC extract reversed ovariectomy-induced deterioration of bone mineral density and bone microarchitecture as well as downregulated cathepsin K (Ctsk) and upregulated runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) in the tibia of OVX rats. Its protective effects on bone were correlated with changes in microbial richness and the restorations of several genera. EC increased the serum circulating levels of acetate and propionate in OVX rats. We conclude that the bone protective effects of EC extract were associated with the changes in microbial compositions and serum short chain fatty acids (SCFAs) in OVX rats.

KW - Erythrinae cortex

KW - Gut microbiota

KW - Ovariec-tomy rats

KW - Prenylated flavonoids

KW - Short chain fatty acids

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U2 - 10.3390/nu13092943

DO - 10.3390/nu13092943

M3 - Journal article

AN - SCOPUS:85113326999

SN - 2072-6643

VL - 13

JO - Nutrients

JF - Nutrients

IS - 9

M1 - 2943

ER -

Prenylated isoflavonoids-rich extract of erythrinae cortex exerted bone protective effects by modulating gut microbial compositions and metabolites in ovariectomized rats

Abstract

Keywords

ASJC Scopus subject areas

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