Precise Ultrasound Neuromodulation in a Deep Brain Region Using Nano Gas Vesicles as Actuators

Xuandi Hou, Zhihai Qiu, Quanxiang Xian, Shashwati Kala, Jianing Jing, Kin Fung Wong, Jiejun Zhu, Jinghui Guo, Ting Zhu, Minyi Yang, Lei Sun

Research output: Journal article publicationJournal articleAcademic researchpeer-review

3 Citations (Scopus)


Ultrasound is a promising new modality for non-invasive neuromodulation. Applied transcranially, it can be focused down to the millimeter or centimeter range. The ability to improve the treatment's spatial resolution to a targeted brain region could help to improve its effectiveness, depending upon the application. The present paper details a neurostimulation scheme using gas-filled nanostructures, gas vesicles (GVs), as actuators for improving the efficacy and precision of ultrasound stimuli. Sonicated primary neurons display dose-dependent, repeatable Ca2+ responses, closely synced to stimuli, and increased nuclear expression of the activation marker c-Fos in the presence of GVs. GV-mediated ultrasound triggered rapid and reversible Ca2+ responses in vivo and could selectively evoke neuronal activation in a deep-seated brain region. Further investigation indicate that mechanosensitive ion channels are important mediators of this effect. GVs themselves and the treatment scheme are also found not to induce significant cytotoxicity, apoptosis, or membrane poration in treated cells. Altogether, this study demonstrates a simple and effective method to achieve enhanced and better-targeted neurostimulation with non-invasive low-intensity ultrasound.

Original languageEnglish
Article number2101934
JournalAdvanced Science
Issue number21
Publication statusPublished - 21 Sep 2021


  • low-frequency ultrasound
  • nano gas vesicles
  • transcranial ultrasound stimulation
  • ultrasonic neuromodulation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Chemical Engineering(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Engineering(all)
  • Physics and Astronomy(all)


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