TY - JOUR
T1 - Polymorphic calcium alginate microfibers assembled using a programmable microfluidic field for cell regulation
AU - Huang, Qiwei
AU - Li, Yingyi
AU - Fan, Longfei
AU - Xin, John H.
AU - Yu, Hui
AU - Ye, Dongdong
N1 - Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2020/9/7
Y1 - 2020/9/7
N2 - Effectively guiding and accurately controlling cell adhesion and growth on the surfaces of specific morphological materials are key issues and hot research topics for optimizing biomaterials. Herein, novel polymorphic alginate microfibers formed through microfluidic spinning technology in a single microchip are presented. Through programming the flow and reaction kinetics in microchannels, other than self-modified micromorphic channel geometry, polymorphic microfibers with precisely tuned curvature-adjustable morphology can be obtained. Finite element (FE) simulations of the flow field (unidirectional fluid-solid coupling) proved the efficacy of the proposed control strategy. Moreover, the specific disordered-ordered cell arrangements showed a linear relationship between bioinspired alginate microfibers with different curvatures and the orientation angle of L929 cells, and diversified growth morphologies, including oblate ellipse, star, tree and strip shapes, occurred on the customizable interface curvature of the calcium alginate microfibers, providing a paradigm for using specific structured natural biomedical materials for cell regulation. This work represents a new design concept for manufacturing polymorphic fibrous biomedical materials through a unique marriage of the fields of green chemistry, hydromechanics, and biomaterials, which should be very useful for guiding the controllable construction of alginate materials for use in structural materials for biomedical and engineering purposes. This journal is
AB - Effectively guiding and accurately controlling cell adhesion and growth on the surfaces of specific morphological materials are key issues and hot research topics for optimizing biomaterials. Herein, novel polymorphic alginate microfibers formed through microfluidic spinning technology in a single microchip are presented. Through programming the flow and reaction kinetics in microchannels, other than self-modified micromorphic channel geometry, polymorphic microfibers with precisely tuned curvature-adjustable morphology can be obtained. Finite element (FE) simulations of the flow field (unidirectional fluid-solid coupling) proved the efficacy of the proposed control strategy. Moreover, the specific disordered-ordered cell arrangements showed a linear relationship between bioinspired alginate microfibers with different curvatures and the orientation angle of L929 cells, and diversified growth morphologies, including oblate ellipse, star, tree and strip shapes, occurred on the customizable interface curvature of the calcium alginate microfibers, providing a paradigm for using specific structured natural biomedical materials for cell regulation. This work represents a new design concept for manufacturing polymorphic fibrous biomedical materials through a unique marriage of the fields of green chemistry, hydromechanics, and biomaterials, which should be very useful for guiding the controllable construction of alginate materials for use in structural materials for biomedical and engineering purposes. This journal is
UR - http://www.scopus.com/inward/record.url?scp=85090070105&partnerID=8YFLogxK
U2 - 10.1039/d0lc00517g
DO - 10.1039/d0lc00517g
M3 - Journal article
C2 - 32696776
AN - SCOPUS:85090070105
SN - 1473-0197
VL - 20
SP - 3158
EP - 3166
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
IS - 17
ER -