Poly(L-lactide)/multiwalled carbon nanotube composites: Interaction with osteoblast-like cells in vitro

Brian Yu Fung Pow, Arthur Fuk Tak Mak, Sau Wong Man, Mo Yang

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

4 Citations (Scopus)

Abstract

In this work, the effect of multiwalled carbon nanotubes (NWNTs) incorporation in poly(L-lactide) (PLLA) on the human osteoblast-like cell (Saos-2) growth behaviour was studied. The PLLA/MWCNT composite with different ratio of PLLA to MWCNT was fabricated using solvent-casting technique. MWCNT was detected by XRD and showed the successful incorporation of MWCNT into PLLA. Fixed amount of cells were cultured on different composite. Cell viability assay and scanning electron microscopy were used to evaluate cell adhesion, morphology and cytoskeleton development on the scaffolds. The activity of mitochondrial dehydrogenase (MD) enzyme was evaluated by MTT substrate and p-nitrophenyl phosphate substrate. The results demonstrated cytoskeleton fibre and good adhesion of osteoblast cells to the composite surface. No significant differences of cell viability were found for the PLLA/MWCNT composite and controls. The incorporation of MWCNT into PLLA can obviously enhance the MD activity. We believe that sample with more MWCNT provided favourite interactions to the human osteoblast-like cells, which indicate a potential candidate in bone tissue engineering.
Original languageEnglish
Title of host publicationMulti-functional Materials and Structures - International Conference on Multifunctional Materials and Structures
PublisherTrans Tech Publications
Pages1347-1350
Number of pages4
ISBN (Print)0878493786, 9780878493784
Publication statusPublished - 1 Jan 2008
EventMulti-functional Materials and Structures - International Conference on Multifunctional Materials and Structures - Hong Kong, P.R., Hong Kong
Duration: 28 Jul 200831 Jul 2008

Publication series

NameAdvanced Materials Research
Volume47-50 PART 2
ISSN (Print)1022-6680

Conference

ConferenceMulti-functional Materials and Structures - International Conference on Multifunctional Materials and Structures
Country/TerritoryHong Kong
CityHong Kong, P.R.
Period28/07/0831/07/08

Keywords

  • Multiwalled carbon nanotube
  • Osteoblast cells
  • Poly(L-lactide) (PLLA)

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint

Dive into the research topics of 'Poly(L-lactide)/multiwalled carbon nanotube composites: Interaction with osteoblast-like cells in vitro'. Together they form a unique fingerprint.

Cite this