PLLA scaffolds with biomimetic apatite coating and biomimetic apatite/collagen composite coating to enhance osteoblast-like cells attachment and activity

Yun Chen, Arthur F.T. Mak, Min Wang, Jiashen Li, Man Sau Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

101 Citations (Scopus)


Novel scaffolds composed of poly(l-lactic acid) (PLLA) skeleton covered with bonelike apatite or apatite/collagen composite were produced via a combined phase-separation technique and an accelerated biomimetic coating process. Saos-2 osteoblast-like cells were used to evaluate the cellular behaviors on these biomimetic coatings. Cell morphologies on the surfaces of PLLA scaffolds, PLLA scaffolds with apatite coating, and PLLA scaffolds with apatite/collagen composite coating were studied by scanning electron microscopy (SEM). Cell viability was assessed by the MTT assay. Differentiated osteoblastic function was monitored by measuring alkaline phosphatase activity. These results suggested that the apatite coating and apatite/collagen composite coating generated through the accelerated biomimetic processes could improve the interactions between osteoblasts and the polymeric scaffolds. The apatite/collagen composite coating was more effective than apatite coating in improving such interactions. The PLLA scaffold with apatite/collagen composite coating is promising as a candidate 3D substrate for bone tissue engineering.
Original languageEnglish
Pages (from-to)575-580
Number of pages6
JournalSurface and Coatings Technology
Issue number3-4
Publication statusPublished - 5 Oct 2006


  • Apatite
  • Bone tissue engineering
  • Cell culture
  • Collagen
  • Osteoblast
  • Polylactic acid

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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