TY - JOUR
T1 - Plasmid-mediated ciprofloxacin, carbapenem and colistin resistance of a foodborne Escherichia coli isolate
AU - Liu, Xiaobo
AU - Li, Ruichao
AU - Chan, Edward Wai Chi
AU - Xia, Xiaodong
AU - Chen, Sheng
N1 - Funding Information:
This work was supported by the Basic Research Fund of Shen-zhen ( 20170410160041091 ), the National Key R&D Program of China ( 2018YFD0500300 ) and Education Department of Scientific Research Project of Hunan Province ( 21C0147 ).
Publisher Copyright:
© 2022
PY - 2022/7
Y1 - 2022/7
N2 - Multidrug resistant (MDR) Escherichia coli poses great threat to human and animal health. To investigate the genetic characteristics of conjugative plasmids that encode ciprofloxacin, carbapenem and colistin resistance in a MDR E. coli strain (974) isolated from pork samples in China, the E. coli strain 974 was subjected to conjugation study, S1-PFGE, Southern hybridization and was sequenced by Illumina Nextseq 500 and MinION Platforms. The ciprofloxacin-resistance-encoding plasmid, p974-IncF, was found to harbor multiple mobile elements that in turn contain genes resistant to β-lactams, quinolone, florephenicol, tetracycline, aminoglycosides, sulfonamides, streptomycin, trimethoprim and chloramphenicol. The backbone of blaNDM-1-bearing plasmid, p974-NDM, was found consist of a 17.3kb resistance region and a 56.7kb of transfer region. However, the classical ISCR1 mobile element and class I integron were missing in plasmid p974-NDM. Resembling the backbone of other IncI2 plasmids, the mcr-1-bearing plasmid, p974-MCR, contained genes that encode replication function, plasmid stability, proteins related to type IV secretion complex, as well as two pilus-encoding genes, which are conserved among all IncI2 plasmids. Enhanced surveillance efforts and limitation of usage of antibiotics are urgently warranted to control the dissemination of these resistance determinants.
AB - Multidrug resistant (MDR) Escherichia coli poses great threat to human and animal health. To investigate the genetic characteristics of conjugative plasmids that encode ciprofloxacin, carbapenem and colistin resistance in a MDR E. coli strain (974) isolated from pork samples in China, the E. coli strain 974 was subjected to conjugation study, S1-PFGE, Southern hybridization and was sequenced by Illumina Nextseq 500 and MinION Platforms. The ciprofloxacin-resistance-encoding plasmid, p974-IncF, was found to harbor multiple mobile elements that in turn contain genes resistant to β-lactams, quinolone, florephenicol, tetracycline, aminoglycosides, sulfonamides, streptomycin, trimethoprim and chloramphenicol. The backbone of blaNDM-1-bearing plasmid, p974-NDM, was found consist of a 17.3kb resistance region and a 56.7kb of transfer region. However, the classical ISCR1 mobile element and class I integron were missing in plasmid p974-NDM. Resembling the backbone of other IncI2 plasmids, the mcr-1-bearing plasmid, p974-MCR, contained genes that encode replication function, plasmid stability, proteins related to type IV secretion complex, as well as two pilus-encoding genes, which are conserved among all IncI2 plasmids. Enhanced surveillance efforts and limitation of usage of antibiotics are urgently warranted to control the dissemination of these resistance determinants.
KW - Carbapenem
KW - Ciprofloxacin
KW - Colistin
KW - Foodborne E. coli
KW - Plasmid
UR - http://www.scopus.com/inward/record.url?scp=85125694354&partnerID=8YFLogxK
U2 - 10.1016/j.foodcont.2022.108937
DO - 10.1016/j.foodcont.2022.108937
M3 - Journal article
AN - SCOPUS:85125694354
SN - 0956-7135
VL - 137
JO - Food Control
JF - Food Control
M1 - 108937
ER -