Photoelectrochemical immunoassay platform based on MoS2 nanosheets integrated with gold nanostars for neuron-specific enolase assay

Renxi Liu, Yanying Wang, Wingleung Wong, Haiyan Li, Chunya Li

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

MoS2 nanosheets were prepared by exfoliating MoS2 bulk crystals with ultrasonication in N-methylpyrrolidone and were integrated with gold nanostars (AuNS) to fabricate an AuNS/MoS2 nanocomposite. All nanomaterials were characterized by transmission electron microscope, scanning electron microscope, ultraviolet-visible spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. AuNS/MoS2 nanocomposites were coated onto a glassy carbon electrode (GCE) surface to construct a nanointerface for immobilizing neuron-specific enolase antibody (anti-NSE) thus forming a photoelectrochemical immunoassay system. AuNS can significantly promote the photoelectric conversion of MoS2 nanosheets improving the performance for a photoelectrochemical assay. Being illuminated with white light LED and controlling the potential at 0.05 V (vs. SCE), the photocurrent generated from anti-NSE(BSA)/AuNS/MoS2/GCE using 0.15 mol L−1 ascorbic acid as electron donor can be recorded with amperometry and used as an output signal for NSE quantitative assay. Under optimized experimental conditions, the photocurrent variation for the affinity-binding NSE is proportional to the logarithm of NSE concentration in the range 5.0 pg mL-1to 1.5 ng mL−1 with a detection limit of 3.5 pg mL−1 (S/N = 3). The practicability of the PEC immunoassay system was evaluated by determining NSE in clinical serum samples. The recoveries ranged from 93.0 to 103% for the determination of NSE in serum samples with a standard addition method. The PEC immunoassay system possesses good accuracy for determining NSE in real samples. [Figure not available: see fulltext.].

Original languageEnglish
Article number480
JournalMicrochimica Acta
Volume187
Issue number8
DOIs
Publication statusPublished - 1 Aug 2020
Externally publishedYes

Keywords

  • Gold nanostar
  • MoS nanosheet
  • Neuron-specific enolase
  • Photoelectrochemical immunoassay

ASJC Scopus subject areas

  • Analytical Chemistry

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