TY - JOUR
T1 - Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2 Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation
AU - Li, Jinming
AU - Leung, Chris Wai Tung
AU - Wong, Dexter Siu Hong
AU - Xu, Jianbin
AU - Li, Rui
AU - Zhao, Yueyue
AU - Yung, Chris Yu Yee
AU - Zhao, Engui
AU - Tang, Ben Zhong
AU - Bian, Liming
PY - 2019/6/26
Y1 - 2019/6/26
N2 - Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).
AB - Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).
KW - aggregation-induced emission
KW - controlled differentiation
KW - real-time monitoring cell differentiation
KW - stem cell
KW - up-conversion nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85068107600&partnerID=8YFLogxK
U2 - 10.1021/acsami.7b00845
DO - 10.1021/acsami.7b00845
M3 - Journal article
C2 - 28350958
AN - SCOPUS:85068107600
SN - 1944-8244
VL - 11
SP - 22074
EP - 22084
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 25
ER -