Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2 Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation

Jinming Li, Chris Wai Tung Leung, Dexter Siu Hong Wong, Jianbin Xu, Rui Li, Yueyue Zhao, Chris Yu Yee Yung, Engui Zhao, Ben Zhong Tang, Liming Bian

Research output: Journal article publicationJournal articleAcademic researchpeer-review

21 Citations (Scopus)

Abstract

Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).

Original languageEnglish
Pages (from-to)22074-22084
Number of pages11
JournalACS Applied Materials and Interfaces
Volume11
Issue number25
DOIs
Publication statusPublished - 26 Jun 2019
Externally publishedYes

Keywords

  • aggregation-induced emission
  • controlled differentiation
  • real-time monitoring cell differentiation
  • stem cell
  • up-conversion nanoparticles

ASJC Scopus subject areas

  • Materials Science(all)

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