PDZ-Reactive Peptide Activates Ephrin-B Reverse Signaling and Inhibits Neuronal Chemotaxis

Yongsheng Yu, Miao Liu, Tsz Tsun Ng, Feng Huang, Yunyu Nie, Rui Wang, Zhongping Yao, Zigang Li, Jiang Xia

Research output: Journal article publicationJournal articleAcademic researchpeer-review

20 Citations (Scopus)

Abstract

Intracellular reactions on nonenzymatic proteins that activate cellular signals are rarely found. We report one example here that a designed peptide derivative undergoes a nucleophilic reaction specifically with a cytosolic PDZ protein inside cells. This reaction led to the activation of ephrin-B reverse signaling, which subsequently inhibited SDF-1 induced neuronal chemotaxis of human neuroblastoma cells and mouse cerebellar granule neurons. Our work provides direct evidence that PDZ-RGS3 bridges ephrin-B reverse signaling and SDF-1 induced G protein signaling for the first time.
Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalACS Chemical Biology
Volume11
Issue number1
DOIs
Publication statusPublished - 15 Jan 2016

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Medicine(all)

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