Patterned Amyloid Materials Integrating Robustness and Genetically Programmable Functionality

Yingfeng Li, Ke Li, Xinyu Wang, Bolin An, Mengkui Cui, Jiahua Pu, Shicao Wei, Shuai Xue, Haifeng Ye, Yanhua Zhao, Minjie Liu, Zuankai Wang, Chao Zhong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

21 Citations (Scopus)

Abstract

The precise manipulation, localization, and assembly of biological and bioinspired molecules into organized structures have greatly promoted material science and bionanotechnology. Further technological innovation calls for new patternable soft materials with the long-sought qualities of environmental tolerance and functional flexibility. Here, we report a patterned amyloid material (PAM) platform for producing hierarchically ordered structures that integrate these material attributes. This platform, combining soft lithography with generic amyloid monomer inks (consisting of genetically engineered biofilm proteins dissolved in hexafluoroisopropanol), along with methanol-assisted curing, enables the spatially controlled deposition and in situ reassembly of amyloid monomers. The resulting patterned structures exhibit spectacular chemical and thermal stability and mechanical robustness under harsh conditions. The PAMs can be programmed for a vast array of multilevel functionalities, including anchoring nanoparticles, enabling diverse fluorescent protein arrays, and serving as self-supporting porous sheets for cellular growth. This PAM platform will not only drive innovation in biomanufacturing but also broaden the applications of patterned soft architectures in optics, electronics, biocatalysis, analytical regents, cell engineering, medicine, and other areas.

Original languageEnglish
Pages (from-to)8399-8408
Number of pages10
JournalNano Letters
Volume19
Issue number12
DOIs
Publication statusPublished - 11 Dec 2019
Externally publishedYes

Keywords

  • Biofilm
  • genetic engineering
  • patterning
  • soft lithography

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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