Abstract
An auxin response factor 2 gene, MiARF2, was cloned in our previous study [1] from the cotyledon section of mango (Mangifera indica L. cv. Zihua) during adventitious root formation, which shares an 84% amino acid sequence similarity to Arabidopsis ARF2. This study was to examine the effects of over-expression of the full-length MiARF2 open reading frame on the root and hypocotyl growth in Arabidopsis. Phenotype analysis showed that the T3 transgenic lines had about 20-30% reduction in the length of hypocotyls and roots of the seedlings in comparison with the wild-type. The transcription levels of ANT and ARGOS genes which play a role in controlling organ size and cell proliferation in the transgenic seedlings also decreased. Therefore, the inhibited root and hypocotyl growth in the transgenic seedlings may be associated with the down-regulated transcription of ANT and ARGOS by the over-expression of MiARF2. This study also suggests that although MiARF2 only has a single DNA-binding domain (DBD), it can function as other ARF-like proteins containing complete DBD, middle region (MR) and carboxy- terminal dimerization domain (CTD). 2010.
Original language | English |
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Pages (from-to) | 3189-3194 |
Number of pages | 6 |
Journal | Molecular Biology Reports |
Volume | 38 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 Jun 2011 |
Keywords
- Auxin
- Auxin response factor (ARF)
- Hypocotyl
- Root growth
ASJC Scopus subject areas
- Molecular Biology
- Genetics