Oriented and vectorial patterning of cardiac myocyte using a microfluidic dielectrophoresis chip - towards engineered cardiac tissue with controlled macroscopic anisotropy

Mo Yang, C.C. Lim, R. Liao, X. Zhang

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

Recently, the ability to create engineered heart tissues with a preferential cell orientation has gained much interest. Here, we present a novel method to construct a cardiac myocyte tissue-like structure using a combination of dielectrophoresis and electro-orientation via a microfluidic chip. Using the interdigitated-castellated microelectrodes, the induction of a mutually attractive dielectrophoretic force between cardiac myocytes can lead the cells moving close to each other and forming a tissue-like structure with orientation along the AC electric field between the electrode gaps. Both experimental results and theoretical analysis have indicated that a large orientation torque and force can be achieved by choosing an optimal frequency and decreasing the conductivity of the medium to a low level, where the orientation torque weakly depends on the frequency. In this paper, electromechanical experiments were performed to demonstrate the structural and functional anisotropy of the electro-oriented structure.
Original languageEnglish
Title of host publication19th IEEE International Conference on Micro Electro Mechanical Systems, 2006 : MEMS 2006 Istanbul, 22-26 January 2006, Istanbul, Turkey
PublisherIEEE
Pages8-11
Number of pages4
ISBN (Print)0780394755
DOIs
Publication statusPublished - 2006

Publication series

NamePROCEEDINGS, IEEE MICRO ELECTRO MECHANICAL SYSTEMS : INTERNATIONAL WORKSHOP ON MICRO ELECTRO MECHANICAL SYSTEMS
ISSN (Print)1084-6999

Keywords

  • Anisotropic magnetoresistance
  • Cardiac tissue
  • Dielectrophoresis
  • Electric fields
  • Electrodes
  • Frequency
  • Heart
  • Microelectrodes
  • Microfluidics
  • Torque

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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