Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity

Duo Bin Mao, Chao Wen Shi, Jianyong Wu, Chun Ping Xu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

7 Citations (Scopus)

Abstract

The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl2, at pH 6.0 and 28°C. A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined. FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols. GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose. Furthermore, thermogravimetric analysis indicated its degradation temperature (Td) of 189°C. The antioxidant activity of the EPS fraction showed a correlation with the molecular properties. It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
Original languageEnglish
Pages (from-to)1401-1409
Number of pages9
JournalBioprocess and Biosystems Engineering
Volume37
Issue number7
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Antioxidants
  • Daedalea dickinsii
  • Exopolysaccharides
  • NMR
  • Thermogravimetry

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this