Optical fiber LPG biosensor integrated microfluidic chip for ultrasensitive glucose detection

Ming Jie Yin, Bobo Huang, Shaorui Gao, Aping Zhang, Xuesong Ye

Research output: Journal article publicationJournal articleAcademic researchpeer-review

69 Citations (Scopus)


An optical fiber sensor integrated microfluidic chip is presented for ultrasensitive detection of glucose. A long-period grating (LPG) inscribed in a small-diameter single-mode fiber (SDSMF) is employed as an optical refractive-index (RI) sensor. With the layer-by-layer (LbL) selfassembly technique, poly (ethylenimine) (PEI) and poly (acrylic acid) (PAA) multilayer film is deposited on the SDSMF-LPG sensor for both supporting and signal enhancement, and then a glucose oxidase (GOD) layer is immobilized on the outer layer for glucose sensing. A microfluidic chip for glucose detection is fabricated after embedding the SDSMF-LPG biosensor into the microchannel of the chip. Experimental results reveal that the SDSMF-LPG biosensor based on such a hybrid sensing film can ultrasensitively detect glucose concentration as low as 1 nM. After integration into the microfluidic chip, the detection range of the sensor is extended from 2 μM to 10 μM, and the response time is remarkablely shortened from 6 minutes to 70 seconds.
Original languageEnglish
Article number259792
Pages (from-to)2067-2077
Number of pages11
JournalBiomedical Optics Express
Issue number5
Publication statusPublished - 1 May 2016


  • Biological sensing and sensors
  • Biomaterials
  • Fiber optics sensors
  • Gratings

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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