Abstract
An optical fiber sensor integrated microfluidic chip is presented for ultrasensitive detection of glucose. A long-period grating (LPG) inscribed in a small-diameter single-mode fiber (SDSMF) is employed as an optical refractive-index (RI) sensor. With the layer-by-layer (LbL) selfassembly technique, poly (ethylenimine) (PEI) and poly (acrylic acid) (PAA) multilayer film is deposited on the SDSMF-LPG sensor for both supporting and signal enhancement, and then a glucose oxidase (GOD) layer is immobilized on the outer layer for glucose sensing. A microfluidic chip for glucose detection is fabricated after embedding the SDSMF-LPG biosensor into the microchannel of the chip. Experimental results reveal that the SDSMF-LPG biosensor based on such a hybrid sensing film can ultrasensitively detect glucose concentration as low as 1 nM. After integration into the microfluidic chip, the detection range of the sensor is extended from 2 μM to 10 μM, and the response time is remarkablely shortened from 6 minutes to 70 seconds.
| Original language | English |
|---|---|
| Article number | 259792 |
| Pages (from-to) | 2067-2077 |
| Number of pages | 11 |
| Journal | Biomedical Optics Express |
| Volume | 7 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 May 2016 |
Keywords
- Biological sensing and sensors
- Biomaterials
- Fiber optics sensors
- Gratings
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics