Nucleophosmin/B23 regulates PCNA promoter through YY1

Jing J. Weng, Yat Ming Yung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

22 Citations (Scopus)

Abstract

Ectopic over-expression of nucleophosmin/B23 caused a marked up-regulation in the amounts of Yin Yang 1 (YY1) and proliferating cellular nuclear antigen (PCNA) proteins. Transfection with nucleophosmin/B23-targeting siRNA induced a decrease in the intracellular amounts of nucleophosmin/B23, YY1, and PCNA. PCNA expression and its promoter activity were attenuated either by nucleophosmin/B23-siRNA or YY1-siRNA. The ChIP assay showed that positive regulation of PCNA is achieved by binding of YY1 to the initiation site of PCNA promoter. The binding of YY1 to the PCNA promoter and histone H4 acetylation was significantly decreased in nucleophosmin/B23-siRNA-treated cells as compared to control vector-transfected cells. Mutation in YY1 binding site resulted in the loss of PCNA promoter activity and the binding of YY1 to the promoter. The results have indicated that YY1 binds to the initiation site of the PCNA promoter along with histone H4 acetylation, leading to transcriptional activity. We have demonstrated that nucleophosmin/B23 plays an important role in the regulation of PCNA through YY1.
Original languageEnglish
Pages (from-to)826-831
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume335
Issue number3
DOIs
Publication statusPublished - 30 Sep 2005
Externally publishedYes

Keywords

  • Nucleophosmin/B23
  • PCNA
  • Transcriptional regulation
  • YY1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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