Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

Hui Jia Chan, Jing Jei Weng, Yat Ming Yung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

25 Citations (Scopus)

Abstract

The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.
Original languageEnglish
Pages (from-to)396-403
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume333
Issue number2
DOIs
Publication statusPublished - 29 Jul 2005
Externally publishedYes

Keywords

  • Nucleophosmin/B23
  • p53
  • Rev peptide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this