Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

Hui Jia Chan; Jing Jei Weng; Yat Ming Yung

doi:10.1016/j.bbrc.2005.04.176

Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

Hui Jia Chan, Jing Jei Weng, Yat Ming Yung

Research output: Journal article publication › Journal article › Academic research › peer-review

27 Citations (Scopus)

Abstract

The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.

Original language	English
Pages (from-to)	396-403
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	333
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2005.04.176
Publication status	Published - 29 Jul 2005
Externally published	Yes

Keywords

Nucleophosmin/B23
p53
Rev peptide

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.1016/j.bbrc.2005.04.176

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title = "Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53",

abstract = "The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.",

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AU - Chan, Hui Jia

AU - Weng, Jing Jei

AU - Yung, Yat Ming

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Y1 - 2005/7/29

N2 - The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.

AB - The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.

KW - Nucleophosmin/B23

KW - p53

KW - Rev peptide

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DO - 10.1016/j.bbrc.2005.04.176

M3 - Journal article

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SN - 0006-291X

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SP - 396

EP - 403

JO - Biochemical and Biophysical Research Communications

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Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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