Normal and cancer breast epithelial cells endocytosis study of nanoparticles by combined AFM and NSOM microscopy

Yu Zhang; Vahid Yazdanpanah; Mo Yang; Mihrimah Ozkan; Cengiz S. Ozkan

doi:10.1109/NANO.2007.4601358

Normal and cancer breast epithelial cells endocytosis study of nanoparticles by combined AFM and NSOM microscopy

Yu Zhang, Vahid Yazdanpanah, Mo Yang, Mihrimah Ozkan, Cengiz S. Ozkan

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

6 Citations (Scopus)

Abstract

A substantial understanding of nanoparticles recognition and uptake by biological cells and tissues is very important for reaching their fullest potential in biomedical research. In this work, we investigated the cell endocytosis of iron oxide nanoparticles by both normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) using a combination of Atomic Force Microscope (AFM) and Nearfield Scanning Optical Microscopy (NSOM) without the need of fluorescent labeling. This hybrid AFM/NSOM imaging method is capable of simultaneous recording topographic and near-field optical images, which provide complementary information in both morphology and optical analyses on the cellular exterior and interior. The resolution of images was observed to be very close to the size of tip. For both cell types, the iron oxide nanoparticles without targeting agents were found to be actively uptaken by a vesicle-mediated transport via a two-step process: a first step of binding at the cell membrane, followed by an subsequent internalization step.

Original language	English
Title of host publication	2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
Pages	1028-1032
Number of pages	5
DOIs	https://doi.org/10.1109/NANO.2007.4601358
Publication status	Published - 1 Dec 2007
Event	2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007 - Hong Kong, Hong Kong Duration: 2 Aug 2007 → 5 Aug 2007

Conference

Conference	2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
Country/Territory	Hong Kong
City	Hong Kong
Period	2/08/07 → 5/08/07

Keywords

AFM
Cell endocytosis
Iron oxide nanoparticle
NSOM

ASJC Scopus subject areas

Electrical and Electronic Engineering
Atomic and Molecular Physics, and Optics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.1109/NANO.2007.4601358

Cite this

@inproceedings{56f113e6d9cb4dfebc45577989184afe,

title = "Normal and cancer breast epithelial cells endocytosis study of nanoparticles by combined AFM and NSOM microscopy",

abstract = "A substantial understanding of nanoparticles recognition and uptake by biological cells and tissues is very important for reaching their fullest potential in biomedical research. In this work, we investigated the cell endocytosis of iron oxide nanoparticles by both normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) using a combination of Atomic Force Microscope (AFM) and Nearfield Scanning Optical Microscopy (NSOM) without the need of fluorescent labeling. This hybrid AFM/NSOM imaging method is capable of simultaneous recording topographic and near-field optical images, which provide complementary information in both morphology and optical analyses on the cellular exterior and interior. The resolution of images was observed to be very close to the size of tip. For both cell types, the iron oxide nanoparticles without targeting agents were found to be actively uptaken by a vesicle-mediated transport via a two-step process: a first step of binding at the cell membrane, followed by an subsequent internalization step.",

keywords = "AFM, Cell endocytosis, Iron oxide nanoparticle, NSOM",

author = "Yu Zhang and Vahid Yazdanpanah and Mo Yang and Mihrimah Ozkan and Ozkan, {Cengiz S.}",

year = "2007",

month = dec,

day = "1",

doi = "10.1109/NANO.2007.4601358",

language = "English",

isbn = "1424406080",

pages = "1028--1032",

booktitle = "2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings",

note = "2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007 ; Conference date: 02-08-2007 Through 05-08-2007",

}

Zhang, Y, Yazdanpanah, V, Yang, M, Ozkan, M & Ozkan, CS 2007, Normal and cancer breast epithelial cells endocytosis study of nanoparticles by combined AFM and NSOM microscopy. in 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings., 4601358, pp. 1028-1032, 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Hong Kong, Hong Kong, 2/08/07. https://doi.org/10.1109/NANO.2007.4601358

Normal and cancer breast epithelial cells endocytosis study of nanoparticles by combined AFM and NSOM microscopy. / Zhang, Yu; Yazdanpanah, Vahid; Yang, Mo et al.
2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings. 2007. p. 1028-1032 4601358.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - Normal and cancer breast epithelial cells endocytosis study of nanoparticles by combined AFM and NSOM microscopy

AU - Zhang, Yu

AU - Yazdanpanah, Vahid

AU - Yang, Mo

AU - Ozkan, Mihrimah

AU - Ozkan, Cengiz S.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - A substantial understanding of nanoparticles recognition and uptake by biological cells and tissues is very important for reaching their fullest potential in biomedical research. In this work, we investigated the cell endocytosis of iron oxide nanoparticles by both normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) using a combination of Atomic Force Microscope (AFM) and Nearfield Scanning Optical Microscopy (NSOM) without the need of fluorescent labeling. This hybrid AFM/NSOM imaging method is capable of simultaneous recording topographic and near-field optical images, which provide complementary information in both morphology and optical analyses on the cellular exterior and interior. The resolution of images was observed to be very close to the size of tip. For both cell types, the iron oxide nanoparticles without targeting agents were found to be actively uptaken by a vesicle-mediated transport via a two-step process: a first step of binding at the cell membrane, followed by an subsequent internalization step.

AB - A substantial understanding of nanoparticles recognition and uptake by biological cells and tissues is very important for reaching their fullest potential in biomedical research. In this work, we investigated the cell endocytosis of iron oxide nanoparticles by both normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) using a combination of Atomic Force Microscope (AFM) and Nearfield Scanning Optical Microscopy (NSOM) without the need of fluorescent labeling. This hybrid AFM/NSOM imaging method is capable of simultaneous recording topographic and near-field optical images, which provide complementary information in both morphology and optical analyses on the cellular exterior and interior. The resolution of images was observed to be very close to the size of tip. For both cell types, the iron oxide nanoparticles without targeting agents were found to be actively uptaken by a vesicle-mediated transport via a two-step process: a first step of binding at the cell membrane, followed by an subsequent internalization step.

KW - AFM

KW - Cell endocytosis

KW - Iron oxide nanoparticle

KW - NSOM

UR - http://www.scopus.com/inward/record.url?scp=52949115694&partnerID=8YFLogxK

U2 - 10.1109/NANO.2007.4601358

DO - 10.1109/NANO.2007.4601358

M3 - Conference article published in proceeding or book

SN - 1424406080

SN - 9781424406081

SP - 1028

EP - 1032

BT - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings

T2 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007

Y2 - 2 August 2007 through 5 August 2007

ER -

Normal and cancer breast epithelial cells endocytosis study of nanoparticles by combined AFM and NSOM microscopy

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

More information

Other files and links

Fingerprint

Cite this