TY - JOUR
T1 - Non-invasive multimodal functional imaging of the intestine with frozen micellar naphthalocyanines
AU - Zhang, Yumiao
AU - Jeon, Mansik
AU - Rich, Laurie J.
AU - Hong, Hao
AU - Geng, Jumin
AU - Zhang, Yin
AU - Shi, Sixiang
AU - Barnhart, Todd E.
AU - Alexandridis, Paschalis
AU - Huizinga, Jan D.
AU - Seshadri, Mukund
AU - Cai, Weibo
AU - Kim, Chulhong
AU - Lovell, Jonathan F.
N1 - Funding Information:
The authors thank L.L. Balos for assistance with histology, C. Cheng for assistance with dynamic light scattering measurements, and E. Huynh and G. Zheng for assistance with photoacoustic spectroscopy. This work was supported by the National Institutes of Health (W.C., R01CA169365; J.F.L., DP5OD017898; M.S., S10OD010393), the Department of Defense (W.C., W81XWH-11-1-0644), the Korean Ministry of Science, ICT and Future Planning (IT Consilience Creative Program; C.K. and J.F.L., NIPA-2013-H0203-13-1001; C.K., NRF-2011-0030075) and a SUNY Research Foundation Collaboration Fund grant.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2014/8
Y1 - 2014/8
N2 - There is a need for safer and improved methods for non-invasive imaging of the gastrointestinal tract. Modalities based on X-ray radiation, magnetic resonance and ultrasound suffer from limitations with respect to safety, accessibility or lack of adequate contrast. Functional intestinal imaging of dynamic gut processes has not been practical using existing approaches. Here, we report the development of a family of nanoparticles that can withstand the harsh conditions of the stomach and intestine, avoid systemic absorption, and provide good optical contrast for photoacoustic imaging. The hydrophobicity of naphthalocyanine dyes was exploited to generate purified ∼ 20nm frozen micelles, which we call nanonaps, with tunable and large near-infrared absorption values (>1,000). Unlike conventional chromophores, nanonaps exhibit non-shifting spectra at ultrahigh optical densities and, following oral administration in mice, passed safely through the gastrointestinal tract. Non-invasive, non-ionizing photoacoustic techniques were used to visualize nanonap intestinal distribution with low background and remarkable resolution, and enabled real-time intestinal functional imaging with ultrasound co-registration. Positron emission tomography following seamless nanonap radiolabelling allowed complementary whole-body imaging.
AB - There is a need for safer and improved methods for non-invasive imaging of the gastrointestinal tract. Modalities based on X-ray radiation, magnetic resonance and ultrasound suffer from limitations with respect to safety, accessibility or lack of adequate contrast. Functional intestinal imaging of dynamic gut processes has not been practical using existing approaches. Here, we report the development of a family of nanoparticles that can withstand the harsh conditions of the stomach and intestine, avoid systemic absorption, and provide good optical contrast for photoacoustic imaging. The hydrophobicity of naphthalocyanine dyes was exploited to generate purified ∼ 20nm frozen micelles, which we call nanonaps, with tunable and large near-infrared absorption values (>1,000). Unlike conventional chromophores, nanonaps exhibit non-shifting spectra at ultrahigh optical densities and, following oral administration in mice, passed safely through the gastrointestinal tract. Non-invasive, non-ionizing photoacoustic techniques were used to visualize nanonap intestinal distribution with low background and remarkable resolution, and enabled real-time intestinal functional imaging with ultrasound co-registration. Positron emission tomography following seamless nanonap radiolabelling allowed complementary whole-body imaging.
UR - http://www.scopus.com/inward/record.url?scp=84905722129&partnerID=8YFLogxK
U2 - 10.1038/nnano.2014.130
DO - 10.1038/nnano.2014.130
M3 - Journal article
C2 - 24997526
AN - SCOPUS:84905722129
SN - 1748-3387
VL - 9
SP - 631
EP - 638
JO - Nature Nanotechnology
JF - Nature Nanotechnology
IS - 8
ER -