Non-invasive, label free, quantitative characterisation of live cells in monolayer culture

Jing Zhang, David Morris, Virginie Sottile, John Crowe, Michael Geoffrey Somekh, Melissa Mather

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review


Cell culture is essential to many areas of biology ranging from the fundamental study of cell biology to the application of cells for therapeutic purposes in Regenerative Medicine. Common to all these areas is the need to characterise cell populations under culture. Currently, cell populations are routinely monitored using conventional biological analysis e.g. cell surface markers, gene expression. This approach is destructive, not suitable for in-process measurements and renders time course experiments impossible. Alternatively non-destructive approaches that assess cell morphology can also be used, with light microscopy techniques (e.g. bright field, phase contrast imaging) being the primary methods. These microscopy techniques can sometimes be combined with the use of exogenous labels such as fluorescent markers. This can provide functional information but has the disadvantage that such cell modifications are invasive and potentially toxic to the cells.
Original languageEnglish
Title of host publication2011 Functional Optical Imaging, FOI 2011
Publication statusPublished - 1 Dec 2011
Externally publishedYes
Event2011 Functional Optical Imaging, FOI 2011 - Ningbo, China
Duration: 3 Dec 20114 Dec 2011


Conference2011 Functional Optical Imaging, FOI 2011

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition

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