Nitric oxide is involved in methyl jasmonate-induced defense responses and secondary metabolism activities of Taxus cells

Wen Wang Jian, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

111 Citations (Scopus)

Abstract

Methyl jasmonate (MeJA), a methyl ester of jasmonic acid (JA), is a well-established signal molecule in plant defense responses and an effective inducer of secondary metabolite accumulation in plant cell cultures such as the valuable anticancer diterpenoid taxol (paclitaxel) in Taxus spp. This work examines the involvement of nitric oxide (NO) in MeJA-induced plant defense responses and secondary metabolism in Taxus chinensis cell cultures. Exogenously supplied MeJA at 100 μM induced rapid production of NO in the Taxus cell cultures, reaching a maximum within 6 h of MeJA supply. Several other responses occurred concomitantly, including the production of hydrogen peroxide (H2O2), and the increases in intracellular malondialdehyde (MDA) content, lipoxygenase (LOX) and phenylalanine ammonium-lyase (PAL) activities. The MeJA-induced H2O2production was suppressed by an NO donor, sodium nitroprusside (SNP), but enhanced by NO inhibitors, Nω-nitro-L-arginine (L-NNA) and 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO). In contrast, the MeJA-induced MDA, LOX and PAL were all enhanced by the NO donor but suppressed by the NO inhibitors. The NO inhibitors also suppressed MeJA-induced taxol accumulation. These results are suggestive of a role for NO as a signal element for activating the MeJA-induced defense responses and secondary metabolism activities of plant cells.
Original languageEnglish
Pages (from-to)923-930
Number of pages8
JournalPlant and Cell Physiology
Volume46
Issue number6
DOIs
Publication statusPublished - 1 Jun 2005

Keywords

  • Methyl jasmonate
  • Nitric oxide
  • Oxidative responses
  • Paclitaxel production
  • Phenylalanine ammonium-lyase
  • Taxus cell culture

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Plant Science
  • Cell Biology

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