Neuroprotective effects of genistein on dopaminergic neurons in the mice model of Parkinson's disease

Li Xing Liu, Wen Fang Chen, Jun Xia Xie, Man Sau Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

86 Citations (Scopus)

Abstract

Emerging evidence suggests beneficial effects of estrogen and estrogen-like chemicals on neurodegenerative diseases, especially Parkinson's disease (PD). Genistein, an isoflavone naturally found in soy products, displays estrogenic properties. The present study aims to investigate the neuroprotective effects of genistein on dopaminergic neurons in ovariectomized (OVX), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD model mice. MPTP significantly decreased the levels of dopamine (DA) and its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the striatum, which could be restored by genistein or estrogen pretreatment. MPTP-challenge with genistein or estrogen pretreatment demonstrated reduced neurotoxicity, with tyrosine hydroxylase-immunoreactive (TH-IR) neurons in the substantia nigra pars compacta (SNpc) affected to a significantly lesser extent as compared to the MPTP treated control. The reverse transcription-PCR results also confirmed that the MPTP-induced downregulation of TH, dopamine transporter (DAT) and Bcl-2 mRNA expression in the midbrain could be restored by genistein or estrogen pretreatment. These findings provide the first evidence that genistein has neuroprotective effects on dopaminergic neurons in the MPTP-induced PD mice and this effect may be attributed to enhancing Bcl-2 gene expression.
Original languageEnglish
Pages (from-to)156-161
Number of pages6
JournalNeuroscience Research
Volume60
Issue number2
DOIs
Publication statusPublished - 1 Feb 2008
Externally publishedYes

Keywords

  • Estrogen
  • Genistein
  • MPTP
  • Parkinson's disease
  • Striatum
  • Substantial nigra

ASJC Scopus subject areas

  • Neuroscience(all)

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