Neuroprotection via inhibition of nitric oxide synthase by bis(7)-tacrine

Wenming Li, Nelson T.K. Lee, Hongjun Fu, Kelvin K.W. Kan, Yuanping Pang, Mingtao Li, Karl W.K. Tsim, Xiaoyuan Li, Yifan Han

Research output: Journal article publicationJournal articleAcademic researchpeer-review

27 Citations (Scopus)

Abstract

Here we report that bis(7)-tacrine, a novel acetylcholinesterase inhibitor, exerts neuroprotective effects by inhibition of nitric oxide synthase. In cortical neurons at 12 days in vitro, bis(7)-tacrine concentration-dependently reduced cell death induced by glutamate, β-amyloid and L-arginine, but not by nitric sodium nitroprusside. NG-monomethyl-L-arginine, a nitric oxide synthase inhibitor, also prevented the former three types but not the last type of the cytotoxicity; however, nitric oxide scavengers blocked all of these insults, indicating that nitric oxide mediated these neuronal injuries. Furthermore, with nitric oxide synthase activity assays, it was found that bis(7)-tacrine not only suppressed the activation of nitric oxide synthase caused by glutamate in cortical neurons, but also directly inhibited the activity of nitric oxide synthase in vitro.
Original languageEnglish
Pages (from-to)471-474
Number of pages4
JournalNeuroReport
Volume17
Issue number5
DOIs
Publication statusPublished - 1 Apr 2006
Externally publishedYes

Keywords

  • Bis(7)-tacrine
  • Neuroprotection
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Neuroscience(all)

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