Nanoporous membrane-based cell chip for the study of anti-cancer drug effect of retinoic acid with impedance spectroscopy

Jinjiang Yu; Zongbin Liu; Mo Yang; Arthur Mak

doi:10.1016/j.talanta.2009.06.047

Nanoporous membrane-based cell chip for the study of anti-cancer drug effect of retinoic acid with impedance spectroscopy

Jinjiang Yu, Zongbin Liu, Mo Yang, Arthur Mak

Research output: Journal article publication › Journal article › Academic research › peer-review

27 Citations (Scopus)

Abstract

This paper presents a novel microchip with nanoporous anodic alumina membrane for the study of anti-cancer drug effect of retinoic acid (RA) on human esophageal squamous epithelial KYSE30 cancer cells in vitro with impedance spectroscopy. The impedance experiments with 0.01 M retinoic acid (RA) were explored for the study of anti-cancer drug effects on KYSE30 cancer cells. The impedance was monitored in the time domain at 0.1 Hz. After addition of 0.01 M RA to the cell chip, the impedance magnitude decreased with time from the value with confluent cell layer and returned to the initial base line after around 12 h. The fluorescence experiments testified that this impedance decrease was due to the cell morphology change induced by RA.

Original language	English
Pages (from-to)	189-194
Number of pages	6
Journal	Talanta
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/j.talanta.2009.06.047
Publication status	Published - 15 Nov 2009

Keywords

Impedance spectroscopy
KYSE30 cancer cells
Nanoporous alumina membrane
Poly(ethylene glycol) (PEG)

ASJC Scopus subject areas

Chemistry(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.1016/j.talanta.2009.06.047

Cite this

@article{4b76c6bf6600499b98f43cf69bd9a902,

title = "Nanoporous membrane-based cell chip for the study of anti-cancer drug effect of retinoic acid with impedance spectroscopy",

abstract = "This paper presents a novel microchip with nanoporous anodic alumina membrane for the study of anti-cancer drug effect of retinoic acid (RA) on human esophageal squamous epithelial KYSE30 cancer cells in vitro with impedance spectroscopy. The impedance experiments with 0.01 M retinoic acid (RA) were explored for the study of anti-cancer drug effects on KYSE30 cancer cells. The impedance was monitored in the time domain at 0.1 Hz. After addition of 0.01 M RA to the cell chip, the impedance magnitude decreased with time from the value with confluent cell layer and returned to the initial base line after around 12 h. The fluorescence experiments testified that this impedance decrease was due to the cell morphology change induced by RA.",

keywords = "Impedance spectroscopy, KYSE30 cancer cells, Nanoporous alumina membrane, Poly(ethylene glycol) (PEG)",

author = "Jinjiang Yu and Zongbin Liu and Mo Yang and Arthur Mak",

year = "2009",

month = nov,

day = "15",

doi = "10.1016/j.talanta.2009.06.047",

language = "English",

volume = "80",

pages = "189--194",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Nanoporous membrane-based cell chip for the study of anti-cancer drug effect of retinoic acid with impedance spectroscopy

AU - Yu, Jinjiang

AU - Liu, Zongbin

AU - Yang, Mo

AU - Mak, Arthur

PY - 2009/11/15

Y1 - 2009/11/15

N2 - This paper presents a novel microchip with nanoporous anodic alumina membrane for the study of anti-cancer drug effect of retinoic acid (RA) on human esophageal squamous epithelial KYSE30 cancer cells in vitro with impedance spectroscopy. The impedance experiments with 0.01 M retinoic acid (RA) were explored for the study of anti-cancer drug effects on KYSE30 cancer cells. The impedance was monitored in the time domain at 0.1 Hz. After addition of 0.01 M RA to the cell chip, the impedance magnitude decreased with time from the value with confluent cell layer and returned to the initial base line after around 12 h. The fluorescence experiments testified that this impedance decrease was due to the cell morphology change induced by RA.

AB - This paper presents a novel microchip with nanoporous anodic alumina membrane for the study of anti-cancer drug effect of retinoic acid (RA) on human esophageal squamous epithelial KYSE30 cancer cells in vitro with impedance spectroscopy. The impedance experiments with 0.01 M retinoic acid (RA) were explored for the study of anti-cancer drug effects on KYSE30 cancer cells. The impedance was monitored in the time domain at 0.1 Hz. After addition of 0.01 M RA to the cell chip, the impedance magnitude decreased with time from the value with confluent cell layer and returned to the initial base line after around 12 h. The fluorescence experiments testified that this impedance decrease was due to the cell morphology change induced by RA.

KW - Impedance spectroscopy

KW - KYSE30 cancer cells

KW - Nanoporous alumina membrane

KW - Poly(ethylene glycol) (PEG)

UR - http://www.scopus.com/inward/record.url?scp=70349246359&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2009.06.047

DO - 10.1016/j.talanta.2009.06.047

M3 - Journal article

C2 - 19782212

SN - 0039-9140

VL - 80

SP - 189

EP - 194

JO - Talanta

JF - Talanta

IS - 1

ER -

Nanoporous membrane-based cell chip for the study of anti-cancer drug effect of retinoic acid with impedance spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

More information

Other files and links

Fingerprint

Cite this