Nanoporous membrane-based cell chip for the study of anti-cancer drug effect of retinoic acid with impedance spectroscopy

Jinjiang Yu, Zongbin Liu, Mo Yang, Arthur Mak

Research output: Journal article publicationJournal articleAcademic researchpeer-review

27 Citations (Scopus)

Abstract

This paper presents a novel microchip with nanoporous anodic alumina membrane for the study of anti-cancer drug effect of retinoic acid (RA) on human esophageal squamous epithelial KYSE30 cancer cells in vitro with impedance spectroscopy. The impedance experiments with 0.01 M retinoic acid (RA) were explored for the study of anti-cancer drug effects on KYSE30 cancer cells. The impedance was monitored in the time domain at 0.1 Hz. After addition of 0.01 M RA to the cell chip, the impedance magnitude decreased with time from the value with confluent cell layer and returned to the initial base line after around 12 h. The fluorescence experiments testified that this impedance decrease was due to the cell morphology change induced by RA.
Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalTalanta
Volume80
Issue number1
DOIs
Publication statusPublished - 15 Nov 2009

Keywords

  • Impedance spectroscopy
  • KYSE30 cancer cells
  • Nanoporous alumina membrane
  • Poly(ethylene glycol) (PEG)

ASJC Scopus subject areas

  • Chemistry(all)

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