Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection

W. W. Ye; J. Y. Shi; C. Y. Chan; L. D. Xiao; Mo Yang

doi:10.1109/Transducers.2013.6627003

Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection

W. W. Ye, J. Y. Shi, C. Y. Chan, L. D. Xiao, Mo Yang

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

3 Citations (Scopus)

Abstract

Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane and nanoparticles based sensing platform has been developed for direct DNA detection. Single stranded DNA (ssDNA) was first covalently immobilized on the nanopore walls via (3-glycidoxypropyl) trimethoxysilane (GPMS). Double strand DNA (dsDNA) hybridization in the nanopores induced blockage in the nanopores, which could be detected by electrochemical impedance spectroscopy (EIS). The blockage signal was then amplified by DNA with gold nanoparticles (AuNPs) and silver catalytic deposition method. This platform is promising for simple, rapid and sensitive DNA detection.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII
Subtitle of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	1255-1258
Number of pages	4
DOIs	https://doi.org/10.1109/Transducers.2013.6627003
Publication status	Published - 1 Dec 2013
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: 16 Jun 2013 → 20 Jun 2013

Conference

Conference	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13

Keywords

DNA detection
electrochemical impedance spectroscopy
nanoparticles
Nanoporous alumina membrane

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

More information

10.1109/Transducers.2013.6627003

Cite this

Ye, W. W., Shi, J. Y., Chan, C. Y., Xiao, L. D., & Yang, M. (2013). Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 1255-1258). Article 6627003 https://doi.org/10.1109/Transducers.2013.6627003

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title = "Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection",

abstract = "Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane and nanoparticles based sensing platform has been developed for direct DNA detection. Single stranded DNA (ssDNA) was first covalently immobilized on the nanopore walls via (3-glycidoxypropyl) trimethoxysilane (GPMS). Double strand DNA (dsDNA) hybridization in the nanopores induced blockage in the nanopores, which could be detected by electrochemical impedance spectroscopy (EIS). The blockage signal was then amplified by DNA with gold nanoparticles (AuNPs) and silver catalytic deposition method. This platform is promising for simple, rapid and sensitive DNA detection.",

keywords = "DNA detection, electrochemical impedance spectroscopy, nanoparticles, Nanoporous alumina membrane",

author = "Ye, {W. W.} and Shi, {J. Y.} and Chan, {C. Y.} and Xiao, {L. D.} and Mo Yang",

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doi = "10.1109/Transducers.2013.6627003",

language = "English",

isbn = "9781467359818",

pages = "1255--1258",

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Ye, WW, Shi, JY, Chan, CY, Xiao, LD & Yang, M 2013, Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6627003, pp. 1255-1258, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6627003

Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection. / Ye, W. W.; Shi, J. Y.; Chan, C. Y. et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 1255-1258 6627003.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

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N2 - Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane and nanoparticles based sensing platform has been developed for direct DNA detection. Single stranded DNA (ssDNA) was first covalently immobilized on the nanopore walls via (3-glycidoxypropyl) trimethoxysilane (GPMS). Double strand DNA (dsDNA) hybridization in the nanopores induced blockage in the nanopores, which could be detected by electrochemical impedance spectroscopy (EIS). The blockage signal was then amplified by DNA with gold nanoparticles (AuNPs) and silver catalytic deposition method. This platform is promising for simple, rapid and sensitive DNA detection.

AB - Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane and nanoparticles based sensing platform has been developed for direct DNA detection. Single stranded DNA (ssDNA) was first covalently immobilized on the nanopore walls via (3-glycidoxypropyl) trimethoxysilane (GPMS). Double strand DNA (dsDNA) hybridization in the nanopores induced blockage in the nanopores, which could be detected by electrochemical impedance spectroscopy (EIS). The blockage signal was then amplified by DNA with gold nanoparticles (AuNPs) and silver catalytic deposition method. This platform is promising for simple, rapid and sensitive DNA detection.

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Ye WW, Shi JY, Chan CY, Xiao LD, Yang M. Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 1255-1258. 6627003 doi: 10.1109/Transducers.2013.6627003

Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection

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