Nanoporous alumina membrane and nanoparticle based microfluidic sensing platform for direct DNA detection

W. W. Ye, J. Y. Shi, C. Y. Chan, L. D. Xiao, Mo Yang

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

3 Citations (Scopus)

Abstract

Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane and nanoparticles based sensing platform has been developed for direct DNA detection. Single stranded DNA (ssDNA) was first covalently immobilized on the nanopore walls via (3-glycidoxypropyl) trimethoxysilane (GPMS). Double strand DNA (dsDNA) hybridization in the nanopores induced blockage in the nanopores, which could be detected by electrochemical impedance spectroscopy (EIS). The blockage signal was then amplified by DNA with gold nanoparticles (AuNPs) and silver catalytic deposition method. This platform is promising for simple, rapid and sensitive DNA detection.
Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII
Subtitle of host publicationThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages1255-1258
Number of pages4
DOIs
Publication statusPublished - 1 Dec 2013
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
Duration: 16 Jun 201320 Jun 2013

Conference

Conference2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/TerritorySpain
CityBarcelona
Period16/06/1320/06/13

Keywords

  • DNA detection
  • electrochemical impedance spectroscopy
  • nanoparticles
  • Nanoporous alumina membrane

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this