N-Trimethylated chitosan coating white adipose tissue vascular-targeting oral nano-system for the enhanced anti-obesity effects of celastrol

Jing Xian, Xuemei Zhong, Qi Huang, Huan Gu, Yixuan Feng, Jiayi Sun, Di Wang, Jingjing Li, Chen Zhang, Yihan Wu (Corresponding Author), Jinming Zhang (Corresponding Author)

Research output: Journal article publicationJournal articleAcademic researchpeer-review

8 Citations (Scopus)

Abstract

Oral nanoparticles (NPs) are more suitable for obesity control compared to NPs administered intravenously, as their convenience increases patient compliance. Herein, we developed an oral nano-system to improve the anti-obesity efficacy of celastrol (Cel). The observed enhanced efficacy was mediated by zein core NPs decorated with adipose-homing peptides that were coated with N-trimethylated chitosan. The optimized Cel/AHP-NPs@TMC exhibited spherical morphology by TEM, as well as narrow size distribution (221.76 ± 6.73 nm) and adequate stability in a gastrointestinal environment. Based on the combined delivery advantages of AHP-NPs@TMC – i.e., improved cellular internalization within Caco-2 cells and enhanced white adipose tissue (WAT) vascular targeting – Cel/AHP-NPs@TMC significantly reduced the body weight, blood lipid levels, adipose inflammation, and WAT distribution in diet-induced obese mice without side-effects. In short, this study provides clear evidence that TMC-based oral NPs can effectively improve celastrol for obesity treatment.

Original languageEnglish
Article number124023
JournalInternational Journal of Biological Macromolecules
Volume236
DOIs
Publication statusPublished - 1 May 2023

Keywords

  • Celastrol
  • Inflammation
  • N-trimethylated chitosan
  • Obesity
  • Oral targeting nanoparticles

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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