N-acetyl-serotonin reduces copper (I) ion-induced lipid peroxidation in bovine retinal homogenates

Andrew W. Siu, Josephine P. Cheung, Chi Ho To, Eric K. Chan, Joe K. Chan, Jesse C. Cheung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

7 Citations (Scopus)

Abstract

Purpose: Chalcosis is an ocular condition caused by penetration injury of copper or its alloy, which leads to extensive ocular inflammation. N-acetyl-serotonin has recently been identified as a potent antioxidant against free radical stress. In this study, we determined the efficacy of N-acetyl-serotonin against the copper (I)-induced retinal lipid peroxidation. Methods: Copper (I)-treated (100 μM) bovine retinal homogenates were incubated with 6 different concentrations (i.e. 0.00, 0.25, 0.50, 1.00, 2.00 and 4.00 mM) of N-acetyl-serotonin or vitamin E. The malondialdehyde level was measured as an index of lipid peroxidation. Results: Copper (I) ions induced a significant dose-dependent increase in malondialdehyde (p=0.007). Co-incubation with N-acetyl-serotonin or vitamin E significantly suppressed the copper (I)-induced malondialdehyde production (p<0.0001). The concentration to inhibit 50% of damage for N-acetyl-serotonin and vitamin E were found to be 1.54 mM and 0.45 mM, respectively. Conclusion: Although N-acetyl-serotonin is only 29% as effective as vitamin E in suppressing the copper (I)-induced lipid peroxidation, the present study supports a pharmacological potential of N-acetyl-serotonin combating free radical oxidative damages in the ocular tissues.
Original languageEnglish
Pages (from-to)69-71
Number of pages3
JournalActa Ophthalmologica Scandinavica
Volume79
Issue number1
DOIs
Publication statusPublished - 31 Jan 2001

Keywords

  • Chalcosis
  • Copper
  • Lipid peroxidation
  • N-acetyl-serotonin
  • Retina
  • Vitamin E

ASJC Scopus subject areas

  • Ophthalmology

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