Mycelial fermentation characteristics and anti-fatigue activities of a Chinese caterpillar fungus, ophiocordyceps sinensis strain Cs-HK1 (Ascomycetes)

Jianyong Wu, Hong Po Leung, Wen Qiang Wang, Chun Ping Xu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

12 Citations (Scopus)


Mycelial fermentation of an Ophiocordyceps sinensis strain Cs-HK1 was carried out in various volumes of stirred-tank fermenters from 1.6-L and 15-L laboratory scale to 2000-L industrial scale. The mycelial growth in most fermenters had a higher rate, due probably to more effcient oxygen supply, than in shake-fasks. The mycelial fermentation was successfully scaled up to 2000-L industrial fermenters, achieving 30 g/L maximum biomass in 5 days. The Cs-HK1 mycelia formed hairy and fuffy pellets in the fermentation medium and the mycelial broth exhibited pseudoplastic rheology following the power law, with the flow behavior index n decreasing from 0.5 to 0.3, and the fow consistency K and the apparent viscosity μa increasing with time and biomass concentration. The mycelial broth containing biomass and extracellular products harvested from 2000-L fermenters was tested for anti-fatigue activities in forced animal swimming experiments. The mycelium hot water extract showed the most significant effects, increasing the swimming endurance of mice up to 100%, and also increasing the glycogen levels and reducing the lactic acid and blood urea nitrogen levels signifcantly. The results demonstrated the feasibility of Cs-HK1 mycelial fermentation for large-scale production of bioactive and medicinal materials.
Original languageEnglish
Pages (from-to)105-114
Number of pages10
JournalInternational Journal of Medicinal Mushrooms
Issue number2
Publication statusPublished - 1 Jan 2014


  • Anti-fatigue activity
  • Medicinal mushrooms and fungi
  • Morphology
  • Mycelial characteristics
  • Ophiocordyceps sinensis
  • Stirred-tank fermenter

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Pharmacology
  • Medicine(all)

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