Abstract
Mycelial fermentation of an Ophiocordyceps sinensis strain Cs-HK1 was carried out in various volumes of stirred-tank fermenters from 1.6-L and 15-L laboratory scale to 2000-L industrial scale. The mycelial growth in most fermenters had a higher rate, due probably to more effcient oxygen supply, than in shake-fasks. The mycelial fermentation was successfully scaled up to 2000-L industrial fermenters, achieving 30 g/L maximum biomass in 5 days. The Cs-HK1 mycelia formed hairy and fuffy pellets in the fermentation medium and the mycelial broth exhibited pseudoplastic rheology following the power law, with the flow behavior index n decreasing from 0.5 to 0.3, and the fow consistency K and the apparent viscosity μa increasing with time and biomass concentration. The mycelial broth containing biomass and extracellular products harvested from 2000-L fermenters was tested for anti-fatigue activities in forced animal swimming experiments. The mycelium hot water extract showed the most significant effects, increasing the swimming endurance of mice up to 100%, and also increasing the glycogen levels and reducing the lactic acid and blood urea nitrogen levels signifcantly. The results demonstrated the feasibility of Cs-HK1 mycelial fermentation for large-scale production of bioactive and medicinal materials.
Original language | English |
---|---|
Pages (from-to) | 105-114 |
Number of pages | 10 |
Journal | International Journal of Medicinal Mushrooms |
Volume | 16 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Jan 2014 |
Keywords
- Anti-fatigue activity
- Medicinal mushrooms and fungi
- Morphology
- Mycelial characteristics
- Ophiocordyceps sinensis
- Stirred-tank fermenter
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Drug Discovery
- Pharmacology
- General Medicine