TY - JOUR
T1 - Multiple antibiotic resistance index (MARI) of human-isolated Salmonella species: a practical bacterial antibiotic surveillance tool
AU - Woh, Pei Yee
AU - Yeung, May Pui Shan
AU - Goggins, William Bernard
N1 - Funding Information:
This work was supported by Chung Chi College Student Helper Award Scheme 2019 (project no. AF00008), Division of Biostatistics from The Chinese University of Hong Kong (project no. 7103510 and 7051838), and Start-up Fund from The Hong Kong Polytechnic University (project no. P0044917).
Publisher Copyright:
© The Author(s) 2023. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved.
PY - 2023/5
Y1 - 2023/5
N2 - Objectives: Drug-resistant Salmonella plays a significant role in disease morbidity and mortality worldwide. The present study aimed to determine the multiple antibiotic resistance index (MARI) of Salmonella isolated from children hospitalized for gastroenteritis in Hong Kong. Methods: Salmonella isolates from stool samples of children aged from 30 days to <5 years were confirmed by using MALDI-TOF MS and subjected to serotyping methods according to the White–Kauffmann–Le Minor scheme. Antimicrobial susceptibility was determined by agar disc diffusion. Results: A total of 101 Salmonella isolates were serogrouped into Group B (n= 46, 45.5%), Group C (n = 9, 9.0%) or Group D (n = 46, 45.5%), and successfully classified into S. Enteritidis (n = 15) and S. Typhimurium (n= 7). Overall Salmonella susceptibilities demonstrated the highest level of resistance to ampicillin (76.2%), ciprofloxacin (54.0%) and tetracycline (61.2%) whereas MDR strains had high resistance toward ampicillin (100%), tetracycline (100%), cotrimoxazole (84.6%), chloramphenicol (83.3%) and ciprofloxacin (83.3%). MARI revealed that 80.2% of Salmonella including all MDR strains (n= 13) had indexes greater than 0.2. Conclusions: The MARI captures a snapshot of a high rate of antibiotic use and resistance in the isolated Salmonella, indicating the urgent need for continuous antimicrobial susceptibility surveillance and control of antibiotic prescription in selecting effective treatments for human diseases.
AB - Objectives: Drug-resistant Salmonella plays a significant role in disease morbidity and mortality worldwide. The present study aimed to determine the multiple antibiotic resistance index (MARI) of Salmonella isolated from children hospitalized for gastroenteritis in Hong Kong. Methods: Salmonella isolates from stool samples of children aged from 30 days to <5 years were confirmed by using MALDI-TOF MS and subjected to serotyping methods according to the White–Kauffmann–Le Minor scheme. Antimicrobial susceptibility was determined by agar disc diffusion. Results: A total of 101 Salmonella isolates were serogrouped into Group B (n= 46, 45.5%), Group C (n = 9, 9.0%) or Group D (n = 46, 45.5%), and successfully classified into S. Enteritidis (n = 15) and S. Typhimurium (n= 7). Overall Salmonella susceptibilities demonstrated the highest level of resistance to ampicillin (76.2%), ciprofloxacin (54.0%) and tetracycline (61.2%) whereas MDR strains had high resistance toward ampicillin (100%), tetracycline (100%), cotrimoxazole (84.6%), chloramphenicol (83.3%) and ciprofloxacin (83.3%). MARI revealed that 80.2% of Salmonella including all MDR strains (n= 13) had indexes greater than 0.2. Conclusions: The MARI captures a snapshot of a high rate of antibiotic use and resistance in the isolated Salmonella, indicating the urgent need for continuous antimicrobial susceptibility surveillance and control of antibiotic prescription in selecting effective treatments for human diseases.
UR - http://www.scopus.com/inward/record.url?scp=85159543919&partnerID=8YFLogxK
U2 - 10.1093/jac/dkad092
DO - 10.1093/jac/dkad092
M3 - Journal article
C2 - 36994589
AN - SCOPUS:85159543919
SN - 0305-7453
VL - 78
SP - 1295
EP - 1299
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 5
ER -