Multiphase flow manipulation for continuous refractive index analysis of single living cell

L. K. Chin, A. Q. Liu, C. S. Lim, P. H. Yap

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

2 Citations (Scopus)

Abstract

This paper reports an integrated microfluidic system for single cell's refractive index measurement. In this system, a serial multiphase flow manipulation is proposed. Comparison to previous methods developed to measure the cell's refractive index in a microfluidic chip, it ensures that each cell is trapped inside a buffer plug and individually passes through the detection area. This method also avoids cell's wastage and measurement failure due to several cells gathered in the detection area. Results show that measured MDCK cells have an average effective refractive index of 1.383 ± 0.001.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages1348-1350
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - Oct 2007
Externally publishedYes
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: 7 Oct 200711 Oct 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Country/TerritoryFrance
CityParis
Period7/10/0711/10/07

Keywords

  • Cell analysis
  • Fiber Bragg grating
  • Multiphase flow
  • Refractometry

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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