Mucin-targeting-aptamer functionalized liposomes for delivery of cyclosporin A for dry eye diseases (Article)

Ka-Ying Wong, Yibo Liu, Liping Zhou, Man Sau Wong, Juewen Liu (Corresponding Author)

Research output: Journal article publicationJournal articleAcademic researchpeer-review

8 Citations (Scopus)

Abstract

Traditional eye drops are convenient to use; however, their effectiveness is limited by their poor retention time and bioavailability in the eyes due to ocular barriers. Therefore, strategies to enhance ocular drug delivery are required. Herein, we constructed a mucin-1 aptamer-functionalized liposome and loaded it with cyclosporin A, a common ocular drug in eye drops used to treat dry eye diseases (DED). Drug encapsulation slightly reduced the liposome size without changing the surface potential of liposomes. Approximately 90% of the cholesterol-modified aptamers were inserted to the liposomes. We evaluated the cytotoxicity, anti-inflammatory effects, cell permeability regulation, and retention time of liposomes in human corneal epithelial cells under dry eye conditions. These results suggest that the aptamer-functionalized liposomes are more efficient as nanocarriers than non-functionalized liposomes and drug-free liposomes. They restore inflammation levels by 1-fold and remain in the cells for up to 24 h. An in vivo study was also performed in a rat DED model, which demonstrated the efficacy of aptamer-functionalized liposomes in restoring tear production and corneal integrity. The present study demonstrated the capability of aptamer-functionalized liposomes in the delivery of ocular drugs for the management of ocular diseases.
Original languageEnglish
Pages (from-to)4684-4694
Number of pages11
JournalJournal of Materials Chemistry B.
Volume11
Issue number21
DOIs
Publication statusPublished - 3 May 2023

Keywords

  • DRUG-DELIVERY
  • OCULAR DELIVERY
  • MANAGEMENT
  • HYPEROSMOLARITY
  • MECHANISM
  • PRODUCTS
  • SYSTEM
  • DESIGN

ASJC Scopus subject areas

  • General Chemistry
  • Biomedical Engineering
  • General Materials Science

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