MPlum-IFP 1.4 fluorescent fusion protein may display förster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging

Liang-ting Lin, Bo Sheng Wang, Jyh Cheng Chen, Chi Hsien Liu, Chien Chou, Shu Jun Chiu, Wen Yi Chang, Ren Shyan Liu, C. Allen Chang, Yi Jang Lee

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)

Abstract

Bacteriophytochrome infrared fluorescent protein (IFP) has a long emission wavelength that is appropriate for detecting pathophysiological effects via near-infrared (NIR) based imaging. However, the brightness and photostability of IFP are suboptimal, although an exogenous supply of biliverdin (BV) IXα is able to enhance these properties. In this study, we fused a far red mPlum fluorescent protein to IFP 1.4 via a linker deoxyribonucleic acid (DNA) sequence encoding eight amino acids. The brightness of mPlum-IFP 1.4 fusion protein at the IFP emission channel was comparable to that of native IFP 1.4 protein when fusion protein and IFP 1.4 were excited by 543 and 633 nm using confocal microscopy, respectively. Visualization of IFP 1.4 fluorescence by excitation of mPlum in mPlum-IFP 1.4 fusion protein is likely to be associated with Forster resonance energy transfer (FRET). The FRET phenomenon was also predicted by acceptor photobleaching using confocal microscopy. Furthermore, the expression of mPlum-IFP 1.4 fusion protein could be detected in cell culture and in xenograft tumors in the absence of BV using in vivo imaging system, although the BV was still essential for detecting native IFP 1.4. Therefore, this innovative fluorescent fusion protein would be useful for NIR-based imaging in vitro and in vivo.
Original languageEnglish
Article number126013
JournalJournal of Biomedical Optics
Volume18
Issue number12
DOIs
Publication statusPublished - 1 Dec 2013
Externally publishedYes

Keywords

  • Förster resonance energy transfer
  • In vivo imaging
  • mPlum-infrared fluorescent protein 1.4 fusion protein
  • Near-infrared fluorescence

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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