Monolithic silica columns with mixed mode of hydrophilic interaction and weak anion-exchange stationary phase for pressurized capillary electrochromatography

Fanggui Ye, Zenghong Xie, Kwok Yin Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

47 Citations (Scopus)

Abstract

A silica-based monolithic column as polar stationary phase is proposed for pressurized CEC (pCEC). The monolithic silica matrix from a sol-gel process was chemically modified by 3-aminopropyltrimethoxysilane to produce a column for hydrophilic interaction applications. The amino groups on the surface of the polar stationary phase generated anodic EOF under acidic conditions and served at the same time as a weak anion-exchanger. The anion solutes such as nucleotides were separated by the mixed mode mechanism, which comprised hydrophilic interaction, weak anion-exchange, and electrophoresis. The influences of buffer concentration and organic modifier content on the separation of nucleotides by pCEC have been investigated. In addition, the monolithic silica columns were also able to separate various polar compounds such as phenols, nucleic acid bases, and nucleosides in the hydrophilic interaction CIEC mode. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)3373-3380
Number of pages8
JournalElectrophoresis
Volume27
Issue number17
DOIs
Publication statusPublished - 1 Sep 2006

Keywords

  • Hydrophilic interaction
  • Monolithic column
  • Pressurized capillary electro-chromatography
  • Sol-gel
  • Stationary phase

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this