Monitoring the toxicity of phenolic chemicals to activated sludge using a novel optical scanning respirometer

Chun Man Chan, Wai Hung Lo, Kwok Yin Wong, Wai Fun Chung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

14 Citations (Scopus)


This paper reports the development of optical scanning respirometer for determining the toxicity of chemicals to activated sludge. The respirometer is used to measure the dissolved oxygen concentration by monitoring the luminescence intensity of ruthenium dye immobilized in a polymer film in contact with the wastewater sample. The intensity is related to the extent of oxygen quenching of luminescence. The toxicity of chemicals can be evaluated by measuring the inhibition on respiration rate of microorganism using the scanning respirometer. The IC50values (concentration of a chemical that exhibits 50% respiration inhibition) of various phenolic chemicals in activated sludge were determined. The performance of this method is compared with other toxicity methods. The experimental results indicate that the reproducibility and sensitivity of this respirometer are reasonably good.
Original languageEnglish
Pages (from-to)1421-1432
Number of pages12
Issue number9
Publication statusPublished - 1 Oct 1999

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

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