Monitoring the intracellular calcium response to a dynamic hypertonic environment

Xiaowen Huang, Wanqing Yue, Dandan Liu, Jianbo Yue, Jiaqian Li, Dong Sun, Mengsu Yang, Zuankai Wang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)

Abstract

The profiling of physiological response of cells to external stimuli at the single cell level is of importance. Traditional approaches to study cell responses are often limited by ensemble measurement, which is challenging to reveal the complex single cell behaviors under a dynamic environment. Here we report the development of a simple microfluidic device to investigate intracellular calcium response to dynamic hypertonic conditions at the single cell level in real-time. Interestingly, a dramatic elevation in the intracellular calcium signaling is found in both suspension cells (human leukemic cell line, HL-60) and adherent cells (lung cancer cell line, A549), which is ascribed to the exposure of cells to the hydrodynamic stress. We also demonstrate that the calcium response exhibits distinct single cell heterogeneity as well as cell-type-dependent responses to the same stimuli. Our study opens up a new tool for tracking cellular activity at the single cell level in real time for high throughput drug screening.

Original languageEnglish
Article number23591
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 23 Mar 2016
Externally publishedYes

ASJC Scopus subject areas

  • General

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