Abstract
This study aims at investigating physical–chemical properties of a water-soluble heteropolysaccharide from Plnatago asiatica L. leaves, and its immunomodulatory effects on macrophages RAW264.7 cells. Hot water-extracted crude polysaccharide from the plant leaves (PLLCP) was fractionated into four fractions by DEAE Sepharose Fast Flow eluted with 0.1–0.5 M NaCl solutions. The most abundant fraction (0.3 M NaCl elution), designated PLLP-2, was identified as a heteropolysaccharide with an average molecular weight (M w) of 3.54 × 10 4 and composed mainly of Gal (34.4%), GalA (36.5%), Ara (10.1%) and Rha (8.4%). PLLP-2 was an acidic polysaccharide exhibiting inflaky curly aggregation with smooth surface. PLLCP and its main subfraction PLLP-2 displayed immunomodulatory activities by stimulating the production of pro-inflammatory cytokines, including TNF-α and IL-1β in macrophage RAW264.7 cells. These results indicate that the main polysaccharide fraction purified from P. asiatica L. leaves is probably pectin, and have potential immunomodulatory function.
Original language | English |
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Pages (from-to) | 1-4 |
Number of pages | 4 |
Journal | Natural Product Research |
DOIs | |
Publication status | Accepted/In press - 1 Feb 2018 |
Keywords
- immunomodulatory activity
- physical–chemical property
- Plantago asiatica L. leaves
- polysaccharide
- RAW264.7 cells
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Plant Science
- Organic Chemistry