Molecular properties and immunomodulatory activities of a water-soluble heteropolysaccharide isolated from Plantago asiatica L. leaves

Jun Yi Yin, Xin Yue Huang, Li Wang, Jian Qi Guo, Ming Yong Xie, Jianyong Wu, Shao Ping Nie

Research output: Journal article publicationJournal articleAcademic researchpeer-review

5 Citations (Scopus)


This study aims at investigating physical–chemical properties of a water-soluble heteropolysaccharide from Plnatago asiatica L. leaves, and its immunomodulatory effects on macrophages RAW264.7 cells. Hot water-extracted crude polysaccharide from the plant leaves (PLLCP) was fractionated into four fractions by DEAE Sepharose Fast Flow eluted with 0.1–0.5 M NaCl solutions. The most abundant fraction (0.3 M NaCl elution), designated PLLP-2, was identified as a heteropolysaccharide with an average molecular weight (M w) of 3.54 × 10 4 and composed mainly of Gal (34.4%), GalA (36.5%), Ara (10.1%) and Rha (8.4%). PLLP-2 was an acidic polysaccharide exhibiting inflaky curly aggregation with smooth surface. PLLCP and its main subfraction PLLP-2 displayed immunomodulatory activities by stimulating the production of pro-inflammatory cytokines, including TNF-α and IL-1β in macrophage RAW264.7 cells. These results indicate that the main polysaccharide fraction purified from P. asiatica L. leaves is probably pectin, and have potential immunomodulatory function.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalNatural Product Research
Publication statusAccepted/In press - 1 Feb 2018


  • immunomodulatory activity
  • physical–chemical property
  • Plantago asiatica L. leaves
  • polysaccharide
  • RAW264.7 cells

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

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