Modulatory effect of interleukin-1β on rat isolated basilar artery contraction

Sai Wang Seto; Yiu Wa Kwan; Sai Ming Ngai

doi:10.1016/j.ejphar.2005.12.038

Modulatory effect of interleukin-1β on rat isolated basilar artery contraction

Sai Wang Seto, Yiu Wa Kwan, Sai Ming Ngai

Research output: Journal article publication › Journal article › Academic research › peer-review

9 Citations (Scopus)

Abstract

An increased level of cytokine interleukin-1 (IL-1) has been detected around the site of stroke. However, the effect of IL-1β on the basilar artery has received little attention. We evaluated the effects of IL-1β on the contractile response of rat isolated basilar artery by measuring isometric tension change. IL-1β (10 ng/ml) and phenylephrine (0.1 nM) markedly enhanced U46619 (30 and 100 nM)-induced basilar artery contraction. The IL-1β-mediated potentiation was partly suppressed by zinc protoporphyrin (3 μM) and was abolished by tetrodotoxin (TTX, 100 nM), (-)-perillic acid (1 μM), PD98059 (0.3 μM), SB203580 (1 μM) and prazosin (1 μM). Our data suggest that IL-1β (10 ng/ml) causes an enhancement of U46619-mediated basilar artery contraction that probably involves TTX-sensitive neuronal release of an α₁-adrenoceptor agonist and activation of p42/p44 and p38 mitogen-activated protein kinases/p21^ras pathways.

Original language	English
Pages (from-to)	238-245
Number of pages	8
Journal	European Journal of Pharmacology
Volume	531
Issue number	1-3
DOIs	https://doi.org/10.1016/j.ejphar.2005.12.038
Publication status	Published - 15 Feb 2006
Externally published	Yes

Keywords

Adrenergic agonist
Interleukin
MAP kinase
Vasoconstriction

ASJC Scopus subject areas

Pharmacology

More information

10.1016/j.ejphar.2005.12.038

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title = "Modulatory effect of interleukin-1β on rat isolated basilar artery contraction",

abstract = "An increased level of cytokine interleukin-1 (IL-1) has been detected around the site of stroke. However, the effect of IL-1β on the basilar artery has received little attention. We evaluated the effects of IL-1β on the contractile response of rat isolated basilar artery by measuring isometric tension change. IL-1β (10 ng/ml) and phenylephrine (0.1 nM) markedly enhanced U46619 (30 and 100 nM)-induced basilar artery contraction. The IL-1β-mediated potentiation was partly suppressed by zinc protoporphyrin (3 μM) and was abolished by tetrodotoxin (TTX, 100 nM), (-)-perillic acid (1 μM), PD98059 (0.3 μM), SB203580 (1 μM) and prazosin (1 μM). Our data suggest that IL-1β (10 ng/ml) causes an enhancement of U46619-mediated basilar artery contraction that probably involves TTX-sensitive neuronal release of an α1-adrenoceptor agonist and activation of p42/p44 and p38 mitogen-activated protein kinases/p21ras pathways.",

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author = "Seto, {Sai Wang} and Kwan, {Yiu Wa} and Ngai, {Sai Ming}",

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N2 - An increased level of cytokine interleukin-1 (IL-1) has been detected around the site of stroke. However, the effect of IL-1β on the basilar artery has received little attention. We evaluated the effects of IL-1β on the contractile response of rat isolated basilar artery by measuring isometric tension change. IL-1β (10 ng/ml) and phenylephrine (0.1 nM) markedly enhanced U46619 (30 and 100 nM)-induced basilar artery contraction. The IL-1β-mediated potentiation was partly suppressed by zinc protoporphyrin (3 μM) and was abolished by tetrodotoxin (TTX, 100 nM), (-)-perillic acid (1 μM), PD98059 (0.3 μM), SB203580 (1 μM) and prazosin (1 μM). Our data suggest that IL-1β (10 ng/ml) causes an enhancement of U46619-mediated basilar artery contraction that probably involves TTX-sensitive neuronal release of an α1-adrenoceptor agonist and activation of p42/p44 and p38 mitogen-activated protein kinases/p21ras pathways.

AB - An increased level of cytokine interleukin-1 (IL-1) has been detected around the site of stroke. However, the effect of IL-1β on the basilar artery has received little attention. We evaluated the effects of IL-1β on the contractile response of rat isolated basilar artery by measuring isometric tension change. IL-1β (10 ng/ml) and phenylephrine (0.1 nM) markedly enhanced U46619 (30 and 100 nM)-induced basilar artery contraction. The IL-1β-mediated potentiation was partly suppressed by zinc protoporphyrin (3 μM) and was abolished by tetrodotoxin (TTX, 100 nM), (-)-perillic acid (1 μM), PD98059 (0.3 μM), SB203580 (1 μM) and prazosin (1 μM). Our data suggest that IL-1β (10 ng/ml) causes an enhancement of U46619-mediated basilar artery contraction that probably involves TTX-sensitive neuronal release of an α1-adrenoceptor agonist and activation of p42/p44 and p38 mitogen-activated protein kinases/p21ras pathways.

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Modulatory effect of interleukin-1β on rat isolated basilar artery contraction

Abstract

Keywords

ASJC Scopus subject areas

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