Abstract
� 2015 Taylor & Francis. The differential steroid production in the theca and granulosa cells in ovary are resulted from unique enzyme expression profiles. Among them, c-fos, a downstream target of mitogen and extracellular signal-regulated kinases (MEK/ERK) signaling, takes part in this compartment. In this study, we investigated the effect of c-fos on the steady-state levels of CYP17 and CYP19 in human ovarian granulosa-like tumor cell line (KGN) by inhibiting MEK/ERK pathway with PD98059. As a result, our finding demonstrated the distinct distribution patterns of CYP17 and CYP19 in KGN. Moreover, the MEK/ERK pathway functions to inhibit the production of CYP17, while enhance the production of CYP19 in granulosa cells, probably involving a c-fos-dependent mechanism. In conclusion, factors such as c-fos may play a crucial role in the down-regulation of CYP17 and up-regulation of CYP19 in granulosa cells, thereby suppressing androstenedione synthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 201-205 |
| Number of pages | 5 |
| Journal | Gynecological Endocrinology |
| Volume | 32 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 3 Mar 2016 |
| Externally published | Yes |
Keywords
- c-fos
- CYP17
- CYP19
- KGN
- MEK/ERK
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Endocrinology
- Obstetrics and Gynaecology
