Modulation of expression of 17-Hydroxylase/17,20 lyase (CYP17) and P450 aromatase (CYP19) by inhibition of MEK1 in a human ovarian granulosa-like tumor cell line

Xiao Huang; Jiewen Jin; Shanmei Shen; Yanjie Xia; Pei Xu; Xiang Zou; Hongwei Wang; Long Yi; Yong Wang; Qian Gao

doi:10.3109/09513590.2015.1106470

Modulation of expression of 17-Hydroxylase/17,20 lyase (CYP17) and P450 aromatase (CYP19) by inhibition of MEK1 in a human ovarian granulosa-like tumor cell line

Xiao Huang, Jiewen Jin, Shanmei Shen, Yanjie Xia, Pei Xu, Xiang Zou, Hongwei Wang, Long Yi, Yong Wang, Qian Gao

Research output: Journal article publication › Journal article › Academic research › peer-review

13 Citations (Scopus)

Abstract

ï¿½ 2015 Taylor & Francis. The differential steroid production in the theca and granulosa cells in ovary are resulted from unique enzyme expression profiles. Among them, c-fos, a downstream target of mitogen and extracellular signal-regulated kinases (MEK/ERK) signaling, takes part in this compartment. In this study, we investigated the effect of c-fos on the steady-state levels of CYP17 and CYP19 in human ovarian granulosa-like tumor cell line (KGN) by inhibiting MEK/ERK pathway with PD98059. As a result, our finding demonstrated the distinct distribution patterns of CYP17 and CYP19 in KGN. Moreover, the MEK/ERK pathway functions to inhibit the production of CYP17, while enhance the production of CYP19 in granulosa cells, probably involving a c-fos-dependent mechanism. In conclusion, factors such as c-fos may play a crucial role in the down-regulation of CYP17 and up-regulation of CYP19 in granulosa cells, thereby suppressing androstenedione synthesis.

Original language	English
Pages (from-to)	201-205
Number of pages	5
Journal	Gynecological Endocrinology
Volume	32
Issue number	3
DOIs	https://doi.org/10.3109/09513590.2015.1106470
Publication status	Published - 3 Mar 2016
Externally published	Yes

Keywords

c-fos
CYP17
CYP19
KGN
MEK/ERK

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology
Obstetrics and Gynaecology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.3109/09513590.2015.1106470

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title = "Modulation of expression of 17-Hydroxylase/17,20 lyase (CYP17) and P450 aromatase (CYP19) by inhibition of MEK1 in a human ovarian granulosa-like tumor cell line",

abstract = "{\"i}¿½ 2015 Taylor & Francis. The differential steroid production in the theca and granulosa cells in ovary are resulted from unique enzyme expression profiles. Among them, c-fos, a downstream target of mitogen and extracellular signal-regulated kinases (MEK/ERK) signaling, takes part in this compartment. In this study, we investigated the effect of c-fos on the steady-state levels of CYP17 and CYP19 in human ovarian granulosa-like tumor cell line (KGN) by inhibiting MEK/ERK pathway with PD98059. As a result, our finding demonstrated the distinct distribution patterns of CYP17 and CYP19 in KGN. Moreover, the MEK/ERK pathway functions to inhibit the production of CYP17, while enhance the production of CYP19 in granulosa cells, probably involving a c-fos-dependent mechanism. In conclusion, factors such as c-fos may play a crucial role in the down-regulation of CYP17 and up-regulation of CYP19 in granulosa cells, thereby suppressing androstenedione synthesis.",

keywords = "c-fos, CYP17, CYP19, KGN, MEK/ERK",

author = "Xiao Huang and Jiewen Jin and Shanmei Shen and Yanjie Xia and Pei Xu and Xiang Zou and Hongwei Wang and Long Yi and Yong Wang and Qian Gao",

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language = "English",

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Modulation of expression of 17-Hydroxylase/17,20 lyase (CYP17) and P450 aromatase (CYP19) by inhibition of MEK1 in a human ovarian granulosa-like tumor cell line. / Huang, Xiao; Jin, Jiewen; Shen, Shanmei et al.
In: Gynecological Endocrinology, Vol. 32, No. 3, 03.03.2016, p. 201-205.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - Modulation of expression of 17-Hydroxylase/17,20 lyase (CYP17) and P450 aromatase (CYP19) by inhibition of MEK1 in a human ovarian granulosa-like tumor cell line

AU - Huang, Xiao

AU - Jin, Jiewen

AU - Shen, Shanmei

AU - Xia, Yanjie

AU - Xu, Pei

AU - Zou, Xiang

AU - Wang, Hongwei

AU - Yi, Long

AU - Wang, Yong

AU - Gao, Qian

PY - 2016/3/3

Y1 - 2016/3/3

N2 - ï¿½ 2015 Taylor & Francis. The differential steroid production in the theca and granulosa cells in ovary are resulted from unique enzyme expression profiles. Among them, c-fos, a downstream target of mitogen and extracellular signal-regulated kinases (MEK/ERK) signaling, takes part in this compartment. In this study, we investigated the effect of c-fos on the steady-state levels of CYP17 and CYP19 in human ovarian granulosa-like tumor cell line (KGN) by inhibiting MEK/ERK pathway with PD98059. As a result, our finding demonstrated the distinct distribution patterns of CYP17 and CYP19 in KGN. Moreover, the MEK/ERK pathway functions to inhibit the production of CYP17, while enhance the production of CYP19 in granulosa cells, probably involving a c-fos-dependent mechanism. In conclusion, factors such as c-fos may play a crucial role in the down-regulation of CYP17 and up-regulation of CYP19 in granulosa cells, thereby suppressing androstenedione synthesis.

AB - ï¿½ 2015 Taylor & Francis. The differential steroid production in the theca and granulosa cells in ovary are resulted from unique enzyme expression profiles. Among them, c-fos, a downstream target of mitogen and extracellular signal-regulated kinases (MEK/ERK) signaling, takes part in this compartment. In this study, we investigated the effect of c-fos on the steady-state levels of CYP17 and CYP19 in human ovarian granulosa-like tumor cell line (KGN) by inhibiting MEK/ERK pathway with PD98059. As a result, our finding demonstrated the distinct distribution patterns of CYP17 and CYP19 in KGN. Moreover, the MEK/ERK pathway functions to inhibit the production of CYP17, while enhance the production of CYP19 in granulosa cells, probably involving a c-fos-dependent mechanism. In conclusion, factors such as c-fos may play a crucial role in the down-regulation of CYP17 and up-regulation of CYP19 in granulosa cells, thereby suppressing androstenedione synthesis.

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KW - CYP17

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KW - MEK/ERK

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JO - Gynecological Endocrinology

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Modulation of expression of 17-Hydroxylase/17,20 lyase (CYP17) and P450 aromatase (CYP19) by inhibition of MEK1 in a human ovarian granulosa-like tumor cell line

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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