MMP2-sensing up-conversion nanoparticle for fluorescence biosensing in head and neck cancer cells

Y.-C. Chan, C.-W. Chen, M.-H. Chan, Y.-C. Chang, W.-M. Chang, L.-H. Chi, H.-M. Yu, Y.-F. Lin, Din-ping Tsai, R.-S. Liu, M. Hsiao

Research output: Journal article publicationJournal articleAcademic researchpeer-review

37 Citations (Scopus)


© 2016 Elsevier B.V.Upconversion nanoparticles (UCNPs) have extensive biological-applications because of their bio-compatibility, tunable optical properties and their ability to be excited by infrared radiation. Matrix metalloproteinases (MMPs) play important roles in extracellular matrix remodelling; they are usually found to significantly increase during cancer progression, and these increases may lead to poor patient survival. In this study, we produced a biosensor that can be recognized by MMP2 and then be unravelled by the attached quencher to emit visible light. We used 3.5-nm gold nanoparticles as a quencher that absorbed emission from UCNPs at a wavelength of 540nm. The biosensor consists of an upconversion nanoparticle, MMP2-recognized polypeptides and quenchers. Here, UCNPs consisting of NaYF4:Yb3+/Er3+ were prepared via a high temperature co-precipitation method while protecting the oleic acid ligand. To improve the biocompatibility and modify the UCNPs with a polypeptide, they were coated with a silica shell and further conjugated with MMP-recognizing polypeptides. The polypeptide has two ends of featuring carboxylic and thiol groups that react with UCNPs and AuNPs, and the resulting nanoparticles were referred to as UCNP@p-Au. According to the in vitro cell viability analysis, UCNP@p-Au exhibited little toxicity and biocompatibility in head and neck cancer cells. Cellular uptake studies showed that the MMP-based biosensor was activated by 980-nm irradiation to emit green light. This MMP-based biosensor may serve as sensitive and specific molecular fluorescent probe in biological-applications.
Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalBiosensors and Bioelectronics
Publication statusPublished - 15 Jun 2016
Externally publishedYes


  • Gold
  • Head and neck cancer
  • MMP2
  • UCNP

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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