Microfluidic CD4+ T-cell counting device using chemiluminescence-based detection

Zuankai Wang, Sau Yin Chin, Curtis D. Chin, John Sarik, Maritza Harper, Jessica Justman, Samuel K. Sia

Research output: Journal article publicationJournal articleAcademic researchpeer-review

79 Citations (Scopus)

Abstract

This letter demonstrates a microfluidic platform for enumerating CD4+ T-lymphocytes from whole blood using chemiluminescence as a detection method. We microfabricated traps in a chamber and coated them with anti-CD4 antibody to isolate CD4+ T-cells. Based on cell surface-bound CD3 antibodies conjugated with horseradish peroxidase, incubation with chemiluminescent substrate produced a current in the photodetector that was proportional to the number of captured CD4+ T-cells. Analyzing 3 μL of whole blood, the platform exhibited high cell-capture efficiency and produced cell counts with high correlation to results obtained from flow cytometry. Compared to other lab-on-a-chip methods for CD4 counting, this method uses an instrument that requires no external light source and no image processing to produce a digitally displayed result only seconds after running the test.

Original languageEnglish
Pages (from-to)36-40
Number of pages5
JournalAnalytical Chemistry
Volume82
Issue number1
DOIs
Publication statusPublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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