Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo

Xueting Wang; Yu Chen; Xintong Zuo; Naiqing Ding; Huijuan Zeng; Xiang Zou; Xiaodong Han

doi:10.1016/j.fct.2013.07.039

Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo

Xueting Wang
, Yu Chen
, Xintong Zuo
, Naiqing Ding
, Huijuan Zeng
, Xiang Zou
, Xiaodong Han

Research output: Journal article publication › Journal article › Academic research › peer-review

38 Citations (Scopus)

Abstract

Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes.

Original language	English
Pages (from-to)	309-317
Number of pages	9
Journal	Food and Chemical Toxicology
Volume	60
DOIs	https://doi.org/10.1016/j.fct.2013.07.039
Publication status	Published - 1 Oct 2013
Externally published	Yes

Keywords

Apoptosis
Microcystin-LR
Mouse
Testicular cells

ASJC Scopus subject areas

Food Science
Toxicology

More information

10.1016/j.fct.2013.07.039

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title = "Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo",

abstract = "Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes.",

keywords = "Apoptosis, Microcystin-LR, Mouse, Testicular cells",

author = "Xueting Wang and Yu Chen and Xintong Zuo and Naiqing Ding and Huijuan Zeng and Xiang Zou and Xiaodong Han",

year = "2013",

month = oct,

day = "1",

doi = "10.1016/j.fct.2013.07.039",

language = "English",

volume = "60",

pages = "309--317",

journal = "Food and Chemical Toxicology",

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TY - JOUR

T1 - Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo

AU - Wang, Xueting

AU - Chen, Yu

AU - Zuo, Xintong

AU - Ding, Naiqing

AU - Zeng, Huijuan

AU - Zou, Xiang

AU - Han, Xiaodong

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes.

AB - Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes.

KW - Apoptosis

KW - Microcystin-LR

KW - Mouse

KW - Testicular cells

UR - https://www.scopus.com/pages/publications/84883034576

U2 - 10.1016/j.fct.2013.07.039

DO - 10.1016/j.fct.2013.07.039

M3 - Journal article

C2 - 23891578

SN - 0278-6915

VL - 60

SP - 309

EP - 317

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

ER -

Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo

Abstract

Keywords

ASJC Scopus subject areas

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