TY - JOUR
T1 - Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo
AU - Wang, Xueting
AU - Chen, Yu
AU - Zuo, Xintong
AU - Ding, Naiqing
AU - Zeng, Huijuan
AU - Zou, Xiang
AU - Han, Xiaodong
PY - 2013/10/1
Y1 - 2013/10/1
N2 - Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes.
AB - Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes.
KW - Apoptosis
KW - Microcystin-LR
KW - Mouse
KW - Testicular cells
UR - http://www.scopus.com/inward/record.url?scp=84883034576&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2013.07.039
DO - 10.1016/j.fct.2013.07.039
M3 - Journal article
C2 - 23891578
SN - 0278-6915
VL - 60
SP - 309
EP - 317
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
ER -