Microcystin (-LR) induced testicular cell apoptosis via up-regulating apoptosis-related genes in vivo

Xueting Wang, Yu Chen, Xintong Zuo, Naiqing Ding, Huijuan Zeng, Xiang Zou, Xiaodong Han

Research output: Journal article publicationJournal articleAcademic researchpeer-review

28 Citations (Scopus)

Abstract

Microcystin-LR (MC-LR) can induce apoptosis of a wide range of tissue cells including testicular cells. The purpose of the study was to find out whether the expression and phosphorylation of p53, Bcl-2 protein family proteins, Cyt c, and caspases were involved in the induction of testicular cell apoptosis by MC-LR in mice. Results showed that following exposure to MC-LR, expression of Bax, caspase 3 and caspase 8 was up-regulated. Significant increases in the phosphorylation of both p53 and Bcl-2 were identified after the administration of MC-LR. The administration of MC-LR also resulted in significant increases of c-myc, c-jun, and c-fos. In conclusion, p53, Bcl-2, Bax, Caspase 3 and Caspase 8 are involved in the regulation of MC-LR-induced apoptosis of testicular cells. The overexpression of c-myc, c-jun and c-fos suggests that MC-LR may have carcinogenic potential for testes.
Original languageEnglish
Pages (from-to)309-317
Number of pages9
JournalFood and Chemical Toxicology
Volume60
DOIs
Publication statusPublished - 1 Oct 2013
Externally publishedYes

Keywords

  • Apoptosis
  • Microcystin-LR
  • Mouse
  • Testicular cells

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this