Microbial community and population dynamics of single-stage autotrophic nitrogen removal for dilute wastewater at the benchmark oxygen rate supply

Yu Tzu Huang, Shiou Shiou Chen, Po Heng Lee, Jaeho Bae

Research output: Journal article publicationJournal articleAcademic researchpeer-review

15 Citations (Scopus)


Microbial communities and their kinetic performance in a single-stage autotrophic nitrogen-removal filter at an optimal oxygen supply were examined to determine the presence and activity of denitrifiers, anaerobic ammonia-oxidizing (anammox), ammonia-oxidizing, and nitrite-oxidizing bacteria. To this end, different molecular biology techniques such as real-time quantitative polymerase chain reaction (qPCR) and biomarkers such as 16S rRNA revealed a diverse microbial community along the filter. It was important to survey the specific species of anammox bacteria using a newly designed Candidatus Brocadiafulgida (BF) specific primer, as well as Candidatus Brocadia anammoxidans (BA) and Candidatus Kuenenia stuttgartiensis (KS) specific primers. An unexpected finding was that the predominant anammox species switched from KS in concentrated wastewater to BA in dilute wastewaters. The Eckenfelder model of the NH3-N transformation along the filter was Se=S0exp(-0.192D/L2.3217). These results provide a foundational understanding of the microbial structure and reaction kinetics in such systems.
Original languageEnglish
Pages (from-to)649-653
Number of pages5
JournalBioresource Technology
Publication statusPublished - 1 Jan 2013


  • Anammox
  • Dilute wastewater
  • Kinetic model
  • Primer
  • Quantitative PCR

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

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