Micro-electrode cell-based biosensor using electrochemical impedance spectroscopy for cancer research

Qingjun Liu, Jinjiang Yu, Hui Yu, Lidan Xiao, Ping Wang, Mo Yang

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

5 Citations (Scopus)

Abstract

In this study, cell biosensor chip with microelectrode arrays was used to monitor the culture behaviour of mammalian cancer cells and evaluate the chemosensitivity of anti-cancer drugs using electrochemical impedance spectroscopy. The electrode arrays were fabricated by semiconductor technology with diameter from 50 μm to 10 μm of each electrode. The human oesophageal cancer cell lines (KYSE 30) were cultured on the surface of the electrode with the help of fibronectin. Morphology changes of cells adhesion, spreading, and proliferation can be detected by impedimetric analysis in a real time and non-invasive way. Anti-caner drug of cisplatin was added to cells for potential drug screening applications. The experimental results show that drug's chemosensitivity effects can be detected by this cell based biosensor.
Original languageEnglish
Title of host publication7th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2008
Pages309-312
Number of pages4
Volume19 IFMBE
DOIs
Publication statusPublished - 1 Dec 2008
Event7th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2008 - Beijing, China
Duration: 22 Apr 200825 Apr 2008

Conference

Conference7th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2008
CountryChina
CityBeijing
Period22/04/0825/04/08

Keywords

  • Anticancer drugs
  • Cancer
  • Cell-based biosensor
  • Chemosensitivity
  • Impedance spectroscopy
  • Micro-electrode arrays

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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