Micro-dissection of rat brain for RNA or protein extraction from specific brain region

Kin Chiu; Wui Man Lau; Ho Tak Lau; Kwok Fai So; Raymond Chuen Chung Chang

doi:10.3791/269

Micro-dissection of rat brain for RNA or protein extraction from specific brain region

Kin Chiu, Wui Man Lau, Ho Tak Lau, Kwok Fai So, Raymond Chuen Chung Chang

Research output: Journal article publication › Journal article › Academic research › peer-review

156 Citations (Scopus)

Abstract

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.

Original language	English
Article number	e269
Journal	Journal of Visualized Experiments
Issue number	7
DOIs	https://doi.org/10.3791/269
Publication status	Published - 1 Aug 2007
Externally published	Yes

Keywords

Brain
Dissection
Issue 7
Neuroscience

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Immunology and Microbiology
General Biochemistry,Genetics and Molecular Biology

More information

10.3791/269

Cite this

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Micro-dissection of rat brain for RNA or protein extraction from specific brain region

Abstract

Keywords

ASJC Scopus subject areas

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