MHC expression kinetics and immunogenicity of mesenchymal stromal cells after short-term IFN-γ challenge

Wing Keung Chan, Allan Sik-Yin Lau, James Chun-Bong Li, Ka Wai Helen Law, Yu Lung Lau, Godfrey Chi-Fung Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

93 Citations (Scopus)

Abstract

Objective: Under the influence of interferon-γ (IFN-γ), mesenchymal stromal cells (MSCs) are conditional antigen-presenting cells, which have immunosuppressive potential. Apart from IFN-γ upregulation of major histocompatibility complexes class I and II (MHC-I and MHC-II) expression, the underlying kinetics and mechanisms have not been described previously. This information is helpful to delineate how human MSCs can be modulated by IFN-γ in different clinical scenarios. Materials and Methods: Here, we demonstrated that IFN-γ-treated MSCs underwent classical signal transduction pathway via phosphorylation of signal transducers and activators of transcription-1, activation of interferon regulatory factor-1, and class II transactivator comparable to that of primary human blood macrophages. Results: IFN-γ markedly induced expression of MHC-I instantly, while its effects on MHC-II were less dramatic and delayed up to 4 days. This is due to a slower intracellular transport of the MHC-II antigen to the membrane surface. More important is that MSCs showed a reduction in their proliferation by 50% without evidence of cell death after prolonged IFN-γ treatment for 8 days. High-dose IFN-γ-treated MSCs (500 U/mL) could initiate T-cell activation as indicated by expression of CD25 and proliferation of allogeneic T cells. Conclusions: The summative IFN-γ effects will adversely affect the immunoprivilege status and lifespan of MSCs.
Original languageEnglish
Pages (from-to)1551-1561
Number of pages11
JournalExperimental Hematology
Volume36
Issue number11
DOIs
Publication statusPublished - 1 Jan 2008
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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