Methods for animal cell immobilization using electrostatic droplet generation

B. Bugarski; J. Smith; Jianyong Wu; M. F.A. Goosen

doi:10.1007/BF00151869

Methods for animal cell immobilization using electrostatic droplet generation

B. Bugarski, J. Smith, Jianyong Wu, M. F.A. Goosen

Research output: Journal article publication › Journal article › Academic research › peer-review

32 Citations (Scopus)

Abstract

A technique for the production of small polymer microbeads (less than 150 μm diam.) utilizing electrostatic spraying is presented. Video and image analysis were performed to reveal details of the mechanism of droplet formation. Microbeads coated with poly-L-lysine were prepared and found to be effective as microcarriers for culturing surface immobilized insect cells to a high cell density (1.55 × 107cells/ml microbeads).

Original language	English
Pages (from-to)	677-682
Number of pages	6
Journal	Biotechnology Techniques
Volume	7
Issue number	9
DOIs	https://doi.org/10.1007/BF00151869
Publication status	Published - 1 Sept 1993
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

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10.1007/BF00151869

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title = "Methods for animal cell immobilization using electrostatic droplet generation",

abstract = "A technique for the production of small polymer microbeads (less than 150 μm diam.) utilizing electrostatic spraying is presented. Video and image analysis were performed to reveal details of the mechanism of droplet formation. Microbeads coated with poly-L-lysine were prepared and found to be effective as microcarriers for culturing surface immobilized insect cells to a high cell density (1.55 × 107cells/ml microbeads).",

author = "B. Bugarski and J. Smith and Jianyong Wu and Goosen, {M. F.A.}",

year = "1993",

month = sep,

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doi = "10.1007/BF00151869",

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journal = "Biotechnology Letters",

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AU - Bugarski, B.

AU - Smith, J.

AU - Wu, Jianyong

AU - Goosen, M. F.A.

PY - 1993/9/1

Y1 - 1993/9/1

N2 - A technique for the production of small polymer microbeads (less than 150 μm diam.) utilizing electrostatic spraying is presented. Video and image analysis were performed to reveal details of the mechanism of droplet formation. Microbeads coated with poly-L-lysine were prepared and found to be effective as microcarriers for culturing surface immobilized insect cells to a high cell density (1.55 × 107cells/ml microbeads).

AB - A technique for the production of small polymer microbeads (less than 150 μm diam.) utilizing electrostatic spraying is presented. Video and image analysis were performed to reveal details of the mechanism of droplet formation. Microbeads coated with poly-L-lysine were prepared and found to be effective as microcarriers for culturing surface immobilized insect cells to a high cell density (1.55 × 107cells/ml microbeads).

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U2 - 10.1007/BF00151869

DO - 10.1007/BF00151869

M3 - Journal article

SN - 0141-5492

VL - 7

SP - 677

EP - 682

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 9

ER -

Methods for animal cell immobilization using electrostatic droplet generation

Abstract

ASJC Scopus subject areas

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