Methods for animal cell immobilization using electrostatic droplet generation

B. Bugarski, J. Smith, Jianyong Wu, M. F.A. Goosen

Research output: Journal article publicationJournal articleAcademic researchpeer-review

30 Citations (Scopus)

Abstract

A technique for the production of small polymer microbeads (less than 150 μm diam.) utilizing electrostatic spraying is presented. Video and image analysis were performed to reveal details of the mechanism of droplet formation. Microbeads coated with poly-L-lysine were prepared and found to be effective as microcarriers for culturing surface immobilized insect cells to a high cell density (1.55 × 107cells/ml microbeads).
Original languageEnglish
Pages (from-to)677-682
Number of pages6
JournalBiotechnology Techniques
Volume7
Issue number9
DOIs
Publication statusPublished - 1 Sep 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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